石墨烯作为活细胞生长基底的药效研究

Study on pharmacology efficacy of graphene used as substrates interfacing live cells

分别以盖玻片(Glass),Glass/rGO,Glass/GO为Hela细胞和MDCK细胞生长基底,研究了抗癌药物阿霉素和缺氧试剂FCCP分别诱导Hela和MDCK细胞的凋亡作用。结果表明,相同浓度的药物在Glass基底上均可造成细胞大量死亡;而在Glass/rGO,Glass/GO基底上药物的毒性下降,药效减弱,细胞状态良好;据推测:一是石墨烯对药物的物理吸附作用;二是石墨烯改变了细胞膜的状态而造成的药效降低,具体的机制需要进一步试验来验证。

The pharmacology efficacy of graphene used as substrates interfacing live cells was investigated.Respectively with coverslip（Glass）,Glass/rGO,Glass/GO as the growth substrates interfacing Hela cells and MDCK cells,cell death was studied when Hela and MDCK cells were treated with a dose of the anticancer drug doxorubicin and uncoupling reagent FCCP.The results showed that the same concentration of drugs can cause a large number of cells growing on the glass substrate dead,while the cytotoxicity of drugs weakened for cells growing on the Glass/rGO,Glass/GO substrate.It was assumed that two factors can explain this phenomena,one was that graphene adsorbed drugs physically.Another was that graphene changed cell membrane state,specific protection mechanism required further testing.