愈肾方对阿霉素肾病模型大鼠蛋白尿的影响

Effect of Yushen Formula on Proteinuria in Adriamycin Nephropathy Model Rats

目的观察愈肾方对阿霉素(ADR)肾病模型大鼠的干预作用及其可能机制。方法 50只SD大鼠随机分为空白组、模型组、激素组、愈肾方大剂量组、愈肾方小剂量组,每组10只。除空白组外,其余各组大鼠均尾静脉注射2次(间隔7天)ADR建立肾病模型。末次注射后第3天,激素组给予醋酸泼尼松片9.0mg/kg灌胃,愈肾方大、小剂量组分别给予愈肾方39.6g/kg、19.8g/kg灌胃,空白组和模型组均给予等量饮用水,各组共灌胃21天。比较各组大鼠24h尿量及尿蛋白含量、肾功能、血脂、总蛋白(TP)、肾脏组织内皮素1(ET-1)及肾脏组织病理变化。结果与模型组比较,激素组和愈肾方大、小剂量组24 h尿量增加,愈肾方大、小剂量组尿蛋白含量减少(P<0.05或P<0.01)。与空白组比较,各组大鼠肾功能、血脂均显著增高,TP显著降低(P<0.05或P<0.01)。与空白组比较,模型组大鼠肾组织ET-1染色强度升高,激素组与模型组无显著差异,愈肾方大、小剂量组中ET-1染色强度降低。结论愈肾方可明显降低ADR肾病模型大鼠尿蛋白,可有效修复肾小球结构、足细胞及裂孔隔膜形态,其机理可能与减少ET-1的分泌有关。

Objective To observe the therapeutic effect and possible mechanism of Yushen Formula （formula to treat nephropathy, 愈肾方） for adriamycin nephropathy （AN） in rats. Methods Fifty SD rats were randomized in- to blank group, model group, hormone group and Yushen Formula large-dose group and small-dose groups, with 10 in each. The nephropathy models were established by tail vein injection of adriamycin （ADR） twice （7 days interval） in all groups except the blank group. On the 3rd day after the last injection, the hormone group was gavaged with 9.0 mg/kg prednisone tablets, Yushen Formula large-dose and small-dose groups were gavaged with 39.6 g/kg and 19. 8 g/kg Yushen Formula, and the blank group and model group were gavaged with the same amount of drinking wa- ter for 21 days. The 24 h urine volume and 24 h urinary protein （24 hUP）, renal function, blood lipids, total protein （TP）, kidney tissue endothelin-1 （ET-1） and renal pathological changes were compared in all groups. Results Comparing with the model group, the 24 h urine volumes were significantly increased in the hormone group and Yushen Formula large-dose and small-dose groups, but the 24 hUP was significantly decreased in Yushen Formula large-dose and small-dose groups （ P 〈 0.05 or P 〈 0.01 ）. Comparing with the blank group, the renal function and blood lipids were significantly increased but TP was significantly decreased in other groups （ P 〈 0. 05 or P 〈 0.01 ）. Comparing with the blank group, the staining intensity of kidney tissue ET-1 was significantly increased in the model group but decreased in Yushen Formula large-dose and small-dose groups. Conclusion Yushen Formula can obviously reduce urinary protein and repair glomerular structure, podocyte and slit diaphragm in AN rats. Its mechanism may be associ- ated with reduced secretion of ET-1.