摘要
目的探讨四君子汤联合缺血预处理(ischemia preconditioning,IPC)对大鼠肝脏缺血再灌注损伤的保护作用及机制。方法将SD大鼠100只随机分成假手术组、空白对照组、四君子汤预处理组、IPC组、四君子汤预处理+IPC组。肝脏缺血再灌注术后检测血清天门冬氨酸氨基转移酶(AST)、丙氨酸氨基转移酶(ALT);检测肝细胞原位凋亡;检测肝组织中丙二醛(MDA)和超氧化物歧化酶(SOD)。结果四君子汤预处理+IPC组ALT和AST水平与空白对照组、四君子汤预处理组、IPC组三组比较,明显降低,差异有统计学意义(P<0.05)。四君子汤预处理组及IPC组ALT和AST水平与空白对照组比较,明显降低,差异有统计学意义(P<0.05)。四君子汤预处理组、IPC组、四君子汤预处理+IPC组三组中肝细胞凋亡指数和MDA水平均显著低于空白对照组,差异有统计学意义(P<0.05)。四君子汤预处理+IPC组中SOD水平显著高于其余四组,差异有统计学意义(P<0.05)。四君子汤预处理组、IPC组SOD水平显著高于空白对照组,差异有统计学意义(P<0.05)。结论四君子汤联合IPC,在大鼠肝脏缺血再灌注时减轻过氧化损伤,抑制细胞凋亡,从而起到保护作用。
Objective To investigate the effect of Decoction of Four Mild Drugs with ischemia preconditioning against hepatic is-chemia-reperfusion in rats .Methods 100 SD rats were randomly divided into the SHAM ,the Control ,the Decoction of Four Mild Drugs , the IPC ,the Decoction of Four Mild Drugs + IPC groups .After a Surgery for hepatic ischemia -reperfusion ,the levels of glutamic acid aminotransferase (ALT);Aspartate aminotransferase (AST);apoptosis;malondialdehyde (MDA);superoxide dismutase( SOD ) were mea-sured .Results ALT and AST levels in the the Decoction of Four Mild Drugs+IPC group were significantly lower than those in the Control , the Decoction of Four Mild Drugs and the IPC groups ( P〈0.05 ) .ALT and AST levels in the Decoction of Four Mild Drugs and the IPC groups were significantly lower than that in the control group ( P〈0.05) .Apoptotic index and MDA level in the the Decoction of Four Mild Drugs+IPC ,the Decoction of Four Mild Drugs and the IPC groups were significantly lower than that in the control group ( P〈0.05) .SOD level in the Decoction of Four Mild Drugs +IPC group was significantly higher than those in the other four groups ( P〈0.05 ) .SOD level in the the Decoction of Four Mild Drugs and IPC group were significantly higher than that in the control group ( P〈0 .05) .Conclusions De-coction of Four Mild Drugs combined with IPC could reduce ischemia -reperfusion injury in rats by alleviating the inflammatory reaction and anti-apoptotic effect .
出处
《浙江实用医学》
2014年第2期83-86,共4页
Zhejiang Practical Medicine
基金
浙江省教育厅2011年科研计划项目(Y201120554)
关键词
缺血再灌注损伤
四君子汤
缺血预处理
凋亡
Hepatic ischemia reperfusion injury
Decoction of Four Mild Drugs
ischemia preconditioning
apoptosis