四君子汤联合缺血预处理对大鼠肝脏缺血再灌注损伤保护作用的研究

Protective effects of Decoction of Four Mild Drugs with ischemia preconditioning against hepatic ischemia /reperfusion injury in rats

目的探讨四君子汤联合缺血预处理(ischemia preconditioning,IPC)对大鼠肝脏缺血再灌注损伤的保护作用及机制。方法将SD大鼠100只随机分成假手术组、空白对照组、四君子汤预处理组、IPC组、四君子汤预处理+IPC组。肝脏缺血再灌注术后检测血清天门冬氨酸氨基转移酶(AST)、丙氨酸氨基转移酶(ALT);检测肝细胞原位凋亡;检测肝组织中丙二醛(MDA)和超氧化物歧化酶(SOD)。结果四君子汤预处理+IPC组ALT和AST水平与空白对照组、四君子汤预处理组、IPC组三组比较,明显降低,差异有统计学意义(P<0.05)。四君子汤预处理组及IPC组ALT和AST水平与空白对照组比较,明显降低,差异有统计学意义(P<0.05)。四君子汤预处理组、IPC组、四君子汤预处理+IPC组三组中肝细胞凋亡指数和MDA水平均显著低于空白对照组,差异有统计学意义(P<0.05)。四君子汤预处理+IPC组中SOD水平显著高于其余四组,差异有统计学意义(P<0.05)。四君子汤预处理组、IPC组SOD水平显著高于空白对照组,差异有统计学意义(P<0.05)。结论四君子汤联合IPC,在大鼠肝脏缺血再灌注时减轻过氧化损伤,抑制细胞凋亡,从而起到保护作用。

Objective To investigate the effect of Decoction of Four Mild Drugs with ischemia preconditioning against hepatic is-chemia-reperfusion in rats .Methods 100 SD rats were randomly divided into the SHAM ,the Control ,the Decoction of Four Mild Drugs , the IPC ,the Decoction of Four Mild Drugs ＋ IPC groups .After a Surgery for hepatic ischemia -reperfusion ,the levels of glutamic acid aminotransferase （ALT）;Aspartate aminotransferase （AST）;apoptosis;malondialdehyde （MDA）;superoxide dismutase（ SOD ） were mea-sured .Results ALT and AST levels in the the Decoction of Four Mild Drugs＋IPC group were significantly lower than those in the Control , the Decoction of Four Mild Drugs and the IPC groups （ P〈0.05 ） .ALT and AST levels in the Decoction of Four Mild Drugs and the IPC groups were significantly lower than that in the control group （ P〈0.05） .Apoptotic index and MDA level in the the Decoction of Four Mild Drugs＋IPC ,the Decoction of Four Mild Drugs and the IPC groups were significantly lower than that in the control group （ P〈0.05） .SOD level in the Decoction of Four Mild Drugs ＋IPC group was significantly higher than those in the other four groups （ P〈0.05 ） .SOD level in the the Decoction of Four Mild Drugs and IPC group were significantly higher than that in the control group （ P〈0 .05） .Conclusions De-coction of Four Mild Drugs combined with IPC could reduce ischemia -reperfusion injury in rats by alleviating the inflammatory reaction and anti-apoptotic effect .