羟丙基纤维素液晶的肝素化及其抗凝血性能研究

Study on the blood anticoagulation of heparin-functionalized hydroxypropyl cellulose liquid crystal

羟丙基纤维素(HPC)经过辛酰化和丙烯酰化反应得到一种新型的辛酰化/丙烯酰化的羟丙基纤维素液晶材料(OPPC),然后利用Micheal加成机理在其表面接枝肝素。傅里叶变换红外光谱表明OPPC膜表面已实现肝素化改性,甲苯胺蓝法测得肝素接枝量为9.42μg/cm2;偏光显微镜观察证实肝素化后OPPC膜在室温下仍呈现胆甾型液晶织构;肝素化的OPPC膜亲水性得到改善,而溶血率和血小板粘附实验证明肝素功能化的液晶膜具有良好的血液相容性。

A new liquid crystal material,octanoylated/acryloylated hydroxypropyl cellulose（OPPC）,was synthesized by the combination of octanoylation and acryloylation on hydroxypropyl cellulose（HPC）and then followed by grafting heparin on the substrate surface via Michael addition.Fourier transform infrared spectrum analysis confirmed the successful grafting of heparin that was quantified to be 9.42μg/cm2 by Toluidine blue method.A cholesteric liquid crystal texture was observed for heparinized OPPC films at room temperature by polarizing optical microscopy.The heparinization on the surface could improve the hydrophilicity of OPPC film. The hemolysis rate and platelet adhesion experiments proved that heparin-functionalized OPPC liquid crystal film had excellent blood compatibility.