1-甲基乙内酰脲对SW480细胞作用的研究

Research of SW-480 cells by 1-Methylhydantoin

目的探讨1-甲基乙内酰脲对人结肠癌SW480细胞增殖的抑制作用。方法以体外培养的人结肠癌SW480细胞株为实验研究对象。实验分为对照组（等量PBS）和1-甲基乙内酰脲组（M）。MTT比色法检测细胞增殖抑制率,1-甲基乙内酰脲对SW480细胞增殖的抑制作用：根据文献数据和预实验,将1-甲基乙内酰脲组设8个浓度组（M：0.5 mg/L、5 mg/L、10 mg/L、20 mg/L、50 mg/L、100 mg/L、200 mg/L、500 mg/L）;1-甲基乙内酰脲八个浓度组分别干预人结肠癌SW480细胞24、48及72 h后,MTT法检测1-甲基乙内酰脲对SW480细胞增殖的抑制作用。结果 MTT法检测结果显示：①1-甲基乙内酰脲在5～500 mg/L浓度范围能抑制SW480细胞增殖,增殖抑制率呈浓度递增趋势。各组间两两比较差异有显著性（P〈0.05）。②1-甲基乙内酰脲作用于结肠癌SW480细胞48 h后,对SW480细胞增殖抑制作用最强。结论 1-甲基乙内酰脲在体外对人结肠癌SW480细胞有抑制增殖的作用。

[ Objectives] To analysis the inhibit proliferation and induced apoptosis effects of 1-Methylhydantoin in human colonic cancer cell line SW480. [ Methods ] Cultivated human colon cancer SW480 cells in vitro as the study abject, MTT assay was used to detect the ahsorbance after treatment with 1-Methylhydantoin at different con- centrations （0, 0.5, 5, 10, 20, 50, 100, 200, 500 rag/L） alone or in combination with 5-fluorouracil at different con- centrations （10, 20, 50, 100 mg/L） on the proliferation of SW480 cells for different time （24 h, 48 h, and 72 h）, then the inhibition ratio on SW480 cells was derived. 1-Methylhydantoin and 5-fluorouraci with a special concentration （100 rag/L） respectively effect on SW480 cells ordinary microscope. [Results ] MTF assay results of proliferation in- hibitory rate in experimental groups show that： 1-Methylbydanto could suppress proliferation of SW480 cells within the range of experimental concentrations（0.5, 5, 10, 20, 50, 100, 200, 500 mg/L）, and the function intension depend- ed on the density of 1-Methylhydantoin （P 〈0.05）. 1-Methylhydantoin exhibited a highest inhibition ratio on colonic cancer cell line SW480 after effecting 48 hour. [ Conclusions ] 1-Methylhydantoin could inhibit proliferation and in- duced apoptosis of colon cancer SW480 cells in vitro, blocked cell cycle in G0/G1 phase.