DOG1、CD117和Ki67在胃肠道间质瘤中的表达及其与临床病理因素和危险度的相关性

Expression of DOG1,CD117 and Ki67 in Gastrointestinal Stromal Tumors and Their Correlation with Clinicopathological Features and Risk Rank of GISTs

目的探讨DOG1、CD117和Ki67在胃肠道间质瘤中表达及其与临床病理因素的相关性和危险度的关系。方法应用免疫组化方法检测DOG1、CD117和Ki67在66例GIST中表达情况,并对病例进行危险度分级,分析上述免疫组化指标与临床病理相关因素和危险度之间的关系。结果在66例GIST中,DOG1表达阳性率为93.94%,CD117表达阳性率为65.15%,其中CD117阴性的GIST中DOG1阳性表达率为82.61%。DOG1和CD117阳性表达率与性别、年龄、部位、肿瘤大小、核分裂数、危险度之间差异均无统计学意义（P＞0.05）。但DOG1表达阳性率高于CD117,差异有统计学意义（P〈0.01）。Ki67增殖指数〈1%,1%～5%和＞5%的例数分别为15例、20例、24例。Ki67增殖指数在GIST中阳性表达率为89.39%,Ki67的阳性表达与性别、年龄、部位之间差异无统计学意义（P＞0.05）,而与肿瘤大小、核分裂数及危险度之间差异有统计学意义（P〈0.01）,并且Ki67增殖指数与肿瘤大小、核分裂数及危险度之间均呈正相关（r=0.488、r=0.425、r=0.550）。在CD117阳性的GIST中,Ki67阳性表达率高于DOG1阳性的GIST,两者之间差异无统计学意义（P＞0.05）。结论在GIST中,DOG1比CD117有更高的敏感性,Ki67增殖指数的表达可与肿瘤大小、核分裂数一起作为判断危险度及预测预后的重要指标。

Objective To investigate the expression of DOG1,CD117,and Ki-67 in gastrointestinal stromal tumors and explore their relationship with the clinicopathological factors and risk rank of GISTs. Methods Immunohistochemical method was adopted to detected the expression of DOG1,CD117,and Ki67 in 66 cases of GIST. The risks in all cases were also ranked. The correlation between immunohistochemical indicators and clinicopathological factors and risk ranks of GISTs were analyzed. Results Among the 66 GISTs,the positive rates of DOG1 and CD117 were 93.94% and 65.15%. The positive rate of DOG1 in CD117 negative GISTs was 82.61%. had There were no significant difference among groups in gender,age,position,tumor size,tumor mitotic count in the expression of DOG1 and CD117 and risk rank（P〉0.05）. However,DOG1 was more sensitive than CD117（P〈0.01）. The number of cases with Ki67 proliferation indices 1 %,1%-5% and 5% were 15,20,and 24,respectively. The positive rate of Ki67 was 89.39%. No significant difference was found in the expression of Ki67 among groups in gender,age,and position（P〉0.05）. Nevertheless,e Ki67 protein expression was positively correlated with the tumor size,tumor mitotic count and risk rank（P〈0.01）（r =0.488,r =0.425,r =0.550）. The positive rate of Ki67 was a little bit higher in CD117 positive GISTs than the these DOG1 positive cases,but there was no significant difference（P〉0.05）. Conclusion DOG1 has higher sensitivity than CD117 in GISTs. Ki67protein,tumor size,and tumor mitotic count were risk indicators for GISTs and could be predictors for prognosis.