hsa-miR-197在子宫肌瘤中的表达及生物信息学特征分析

The expression mode of hsa-miR-197 in uterine leiomyomas tissue and related bioinformatics analysis

应用实时定量聚合酶链式反应(PCR)技术检测子宫肌瘤组织中的hsa-miR-197表达水平,并与配对正常子宫肌细胞对比。通过miRbase、UCSC、NCBI等在线数据库获取并分析hsa-miR-197序列保守性及其基因组特征。选择miRanda、MirTarget2及TargetScan三个在线数据库预测hsa-miR-197的靶基因并取交集以便后续分析。通过基因本体(GO)功能注释、GO富集分析和信号转导通路富集分析初步阐明,hsamiR-197靶基因参与调控的细胞功能与信号通路。hsa-miR-197的功能较广泛,参与肿瘤发生的多种生物学过程,提示hsa-miR-197可能与子宫肌瘤的发病机制密切相关。

We performed Real-time PCR to detect the hsa-miR-197 expression levels in uterine leiomyomas tissues and paired normal myometrium separately;online databases including miRbase,UCSC,NCBI are employed to analysis the sequence conservation and genetic characteristics of hsa-miR-197;miRNA databases such as miRanda,MirTarget2 and TargetScan are chosen to predict hsa-miR-197 target genes;meanwhile,the intersection genes of these miRNA databases are chosen,and GO and Pathway analysis of these genes are carried out to explore the potential role of hsa-miR-197 playing in regulating the uterine leiomyomas occurrence and development. hsa-miR-197 functions in broad ground and participates in uterine leiomyomas multiple biology progress,which indicates that hsa-miR-197 could regulate uterine leiomyomas occurrence and development.