上海某区儿童血清轮状病毒LLR型疫苗株的免疫水平

Levels of Immunization to Rotavirus LLR Vaccine Strains among Children in a District of Shanghai

[目的]了解2012年上海部分0～5周岁儿童的血清轮状病毒LLR型中和抗体水平及不同年龄段儿童轮状病毒感染的情况,为疫苗的研究提供科学数据。[方法]在2012年6—8月上海市静安区儿保门诊部就诊的儿童中,筛选无发热、无轮状病毒感染疑似症状的健康儿童血清标本210份,采用微量中和试验方法检测儿童血清对轮状病毒LLR型疫苗株的中和抗体水平,并对检测结果进行统计学分析。[结果]210份0～5周岁儿童血清中,轮状病毒LLR型中和抗体阳性率为27.1%,中和抗体几何平均滴度(GMT)为1∶35.36;0～6个月婴儿血清中和抗体阳性率为16.0%,GMT为1∶28.72;7～11个月儿童血清中和抗体阳性率为2.5%,GMT为1∶25.88。1～2周岁儿童的中和抗体阳性率为20.0%,GMT为1∶30.78,2～3周岁儿童的中和抗体阳性率为20.0%,GMT为1∶32.99,该两年龄组(段)间差异无统计学意义。3～4周岁儿童的中和抗体阳性率为56.67%,GMT为1∶54.84,4～5周岁儿童的中和抗体阳性率为63.33%,GMT为1∶44.55,此两年龄组(段)儿童的阳性率和GMT均显著高于3周岁以下各年龄组,此两组间阳性率差异无统计学意义。[结论]0～3周岁儿童中和抗体水平较低,特别是7～11个月年龄组为最低,提示可能为轮状病毒感染的高危儿童群体。

[ Objective ] To assess the levels of neutralizing antibodies to rotavirus LLR vaccine strains and the infection condition among children aged 0-5 years old in Shanghai, 2012, in the hope of providing the scientific basis for vaccine research. [ Methods ] The rotavirus LLR antibody was detected by neutralization test on the sera specimens of 210 healthy children who visited a child health clinic in Shanghai Jing＇an District from June to August 2012. The obtained data were analyzed by statistical methods. [ Results ] The positive rate of rotavirus LLR antibody among the 210 sera specimens was 27.1%, and the geometric mean titer （GMT） of LLR specific neutralizing antibody was 1：35.36. To be specifiC, the positive rate of rotavirus LLR antibody and the GMTwere 16.0% and 1：28.72 for infants aged 0-6 months, 2.5% and 1：25.88 for children aged 7-11 months, 20.0% and 1：30.78 for children aged 1-2 years, 20.0% and 1：32.99 for children aged 2-3 years, 56.67% and 1：54.84 for children aged 3-4 years, 63.33% and 1：44.55 for children aged 4-5 years. There were no significant differences in the positive rate and the GMT between the 1-2 age group and the 2-3 age group. The 3-4 and the 4-5 age groups reported higher positive rates and GMTs than other age groups. Also, the 3-4 and the 4-5 age groups showed no differences in the positive rate. [ Conclusion ] The level of neutralizing antibodies to LLR strains is low in the children of 0-3 years old, particularly in those aged 7-11 months, indicating that they may be the high-risk population of retavirus.