元胡止痛片UPLC特征图谱的研究

UPLC specific chromatogram of Yuanhu Zhitong tablets

目的建立元胡止痛片UPLC特征图谱测定方法,为元胡止痛片的质量控制提供依据。方法采用Acquity UPLC BEH C18（2.1 mm×100 mm,1.7μm）色谱柱;柱温40℃;检测波长270 nm;流动相为乙腈-0.3mol·L^-1的醋酸铵水溶液梯度洗脱,流速0.3 mL·min^-1,进样量3μL。结果 UPLC特征图谱中主要成分得到有效分离,分析时间较常规液相色谱缩短了1/2～1/3。得到21个共有峰,各批次柴黄片特征图谱与对照图谱相似度均〉0.86,可用于元胡止痛片的定性鉴别。结论首次建立了元胡止痛片UPLC特征图谱的分析方法,此法简单、准确、重复性良好,可用于元胡止痛片的质量控制。

Objective To develop a specific determination method for Yuanhu Zhitong tablets to provide the basis for the quality control of Yuanhu Zhitong tablets. Methods Acquity UPLC BEH C18（2.1 mm×100 mm, 1.7 μm） column was adopted, the column temperature was controlled at 40 ℃, the detected wavelength was 270 nm, The mobile phase was acetonitrile-0.1 mol·L^- 1 ammonium acetate solution at 0.3 mL·min^- 1with gradient elution. The injection volumn was 3 μL. Results The major components in UPLC specific chromatogram had effective segregation with the reduction of analysis time to 1/2- 1/3 compared with HPLC method, and 21 common peaks were obtained as the reference fingerprint. Good similarities with correlation coefficients higher than 0.86 were found in fingerprints between samples and standard fingerprint, which could be utilized for the identification of Yuanhu Zhitong tablets. Conclusion The analysis method for UPLC specific chromatogram of Yuanhu Zhitong tablets is established for the first time, which is accurate, simple and repeatable, and can be used for the quality control of Yuanhu Zhitong tablets.