摘要
目的建立检测中兽药中金刚烷胺残留的UPLC-MS/MS方法。方法样品经提取稀释后,采用ACQUITY UPLCTMBEH C18色谱柱为分离柱,质谱正离子扫描测定。结果金刚烷胺在5～100 ng·mL^-1与峰面积线性关系良好（r^2=0.999 2）;样品检出限为1μg·mL^-1,定量限为2μg·mL^-1。在40、50、60 mg·mL^-1回收率为85%～115%,批内批间变异系数均〈10%。结论本方法快速、灵敏、重现性好,适用于中兽药中非法添加金刚烷胺的检测。
Objective To establish a UPLC-MS/MS method to determine amantadine illegally added in traditional Chinese veterinary medicine. Methods The samples were extracted,and the reconstituted solution was analyzed by UPLC-MS/MS with positive ion mode, with ACQUITY UPLCTMBEH C18 as the analytical column. Results The good linear range was 5- 100 ng · mL^- 1（r^2= 0.999 2）. The detection limit was 1 mg · mL^- 1,and the quantitation limit was 2 mg · mL^- 1. The recovery ranged 85%- 115% and the relative standard deviations were below 10%. Conclusion The method is reliable, sensitive and reproducibilite, suitable for the determination of amantadine residues in traditional Chinese veterinary medicine.
出处
《中南药学》
CAS
2014年第4期363-366,共4页
Central South Pharmacy
基金
山东省自主创新专项(No.2013CXC90202)
