青海弧菌Q67冻干粉急性毒性测试方法研究

Study on the Method of Using Vibrio Qinghaiensis sp. Q67 Freeze-dried Powder to Detect Acute Toxicity

[目的]研究利用青海弧菌Q67冻干粉进行急性毒性测试的方法。[方法]通过一系列试验,探讨青海弧菌Q67冻干粉复苏及与毒物反应接触时间对毒性测试的影响,筛选出适宜青海弧菌急性毒性测试的毒性参照物,在充分借鉴国际标准ISO 11348的基础上分析现行发光抑制率计算方法的不足,提出了优化的计算方法。[结果]青海弧菌Q67冻干粉复苏的30～120 min内进行毒性测试,与毒物的反应时间控制在15～20 min时,测得数据稳定、可靠。ZnCl2适合用作青海弧菌生物急性毒性测试的毒性参照物。[结论]该研究为制定青海弧菌Q67冻干粉急性毒性测试标准提供理论依据。

[Objective] The research aimed to explore the method of using Vibrio qinghaiensis sp. Q67 freeze-dried powder to detect acute toxicity. [Method] Through a series of tests,in this paper,Vibrio qinghaiensis sp. Q67 freeze-dried recovery and toxic reaction time on the influence of the toxicity test was discussed,toxicity reference substance which is suitable for acute toxicity tests with Vibrio qinghaiensis was sifted, and based on full reference to the international standard ISO 11348,analyzing the shortage using current methods to calculate luminescence inhibition rate,optimized methodology was presented. [Result]Test data will be stable and reliable when acute toxicity is detected during 30-120 min from Vibrio qinghaiensis sp. Q67 freeze-dried recovery and toxicity reaction time controls in 15 ～ 20 min. ZnCl2can be used as toxicity reference in acute toxicity tests with Vibrio qinghaiensis sp. Q67. [Conclusion] The study provides the theoretical basis for creating methodological standard of using Vibrio qinghaiensis sp. Q67 freeze-dried powder to detect acute toxicity.