响应面法优化黑木耳多糖与三价铬螯合工艺

Study on the Optimization of Chelating Auricularia auricular Polysaccharides and Chromium( Ⅲ) by Response Surface Methodology

[目的]优化黑木耳多糖和三价铬螯合工艺。[方法]采用分光光度法考察黑木耳多糖与三价铬的质量比、螯合时间、三价铬的初始浓度3个因素对其螯合率的影响,并通过Box-Behnken试验设计对试验数据进行二次响应面分析,优化黑木耳多糖与三价铬的螯合工艺。[结果]试验表明,木耳多糖与三价铬的最佳螯合工艺条件为:木耳多糖与三价铬的质量比为2.2∶1,螯合时间6.18 h,三价铬的初始浓度为5.96 mg/ml,在该条件下螯合率为41.56%。[结论]应用响应面法所得到的黑木耳多糖-铬(Ⅲ)螯合物的合成工艺参数是可行的,可为黑木耳多糖与三价铬螯合物的进一步开发利用提供参考。

[Objective]Optimization of Auricularia auricular polysaccharides and chromium（ Ⅲ） chelate technology. [Method] The effects of three independent variables（ the quality of Auricularia auricular polysaccharides and chromium（ Ⅲ）,chelating time and chromium（ Ⅲ） concentration of initial） with spectrophotometric method. And the chelation process were further optimized by response surface methodology（ RSM） based on a Box-Behnken design. [Result]The results showed that the optimum extraction conditions were as follows： the quality of Auricularia auricular polysaccharides and chromium（ Ⅲ） is 2. 2∶ 1,chelating time is 6. 18 h. Chromium（ Ⅲ） concentration of initial is 5. 96 mg / ml. Under the optimized conditions,chelating rate is 41. 56%. [Conclusion]It is feasible to optimize the chelation processing of Auricularia auricular polysaccharides and chromium（ Ⅲ）. Given that,it could provide several references for further research on detection of it.