桑椹高花色苷及抗氧化能力种质资源的筛选与评价

Screening and Evaluation for High Anthocyanin Content and Antioxidant Activity of Fruit Mulberry Germplasm Resources

以国家桑树种质资源圃华南分圃中保存的160份果桑种质资源为材料,对桑椹高花色苷及抗氧化能力种质资源进行了筛选与评价。结果表明,其总花色苷含量、总抗氧化能力和DPPH清除能力的变幅分别为106.5～1472.0 mg/L、5.4～32.3mmol/mL和33.7%～87.8%,表现出明显的品种间差异。113份二倍体和47份四倍体果桑种质资源桑椹中的花色苷含量、总抗氧化能力和DPPH清除率差异较大,但差异无统计学意义（P＆gt；0.05）。聚类分析表明,160份果桑种质资源可分为6大类群,分别由13、11、56、44、10和26份种质构成。桑椹的总抗氧化能力、DPPH清除率和总花色苷含量呈极显著（P﹤0.01）正相关,表明桑椹的抗氧化能力与其所含的总花色苷类物质密切相关。本研究筛选出了一批高花色苷和抗氧化能力的果桑种质资源,可用于高花色苷和高抗氧化能力果桑新品种的培育。

Germplasms with high anthocyanin content and antioxidant activity in mulberry are important for fruit mulberry breeding. 160 fruit mulberry germplasm resources were screened for evaluation of anthocyanin content and antioxidant activity in this study. The results were summarized as follows,the ranges of anthocyanin content,total antioxidant capacity（ TAC）,1,l-Diphenyl-2-picrylhydrazyl radical（ DPPH） scavenging ability in 160 fruit mulberry accessions were 106. 5-1472. 0 mg / L,5. 4-32. 3 mmol / mL,and 33. 7%-87. 8%,respectively,displaying significant varieties differences. The differences among accessions with ploidies were not significant at 0. 05 levels. 160 fruit mulberry accessions could be clustered into 6 clusters which consisted of 13,11,56,44,10,and 26 accessions, respectively. There were significantly positive correlations between the anthocyanin content and TAC,anthocyanin contents and DPPH. The results showed that total anthocyanin in mulberry were the important antioxidat substances. The accessions with high contents of anthocyanin and antioxidant activity would be expected to be used in further quality breeding of fruit mulberry cultivars.