抗PVY烟草种质的遗传多样性分析

Genetic Diversity Analysis of Tobacco Germplasm Resistance to PVY

利用SSR标记对78份抗PVY烟草种质进行遗传多样性分析,根据类型将78份材料分为烤烟、晒晾烟和黄花烟3个群体。结果表明：（1）从960对引物组合中筛选出45对扩增带型清晰、重复性和多态性好的引物用于78份烟草种质资源遗传多样性研究,共检测到108个位点,其中96个位点具有多态性,多态性比例为88.89%。遗传多样性指数（Nei’s）为0.3766,Shannon’s指数为0.5821,种质间遗传相似系数变异范围在0.0454～0.9973之间,说明我国抗PVY烟草种质资源存在着丰富的遗传变异。（2）3个群体内遗传差异由大到小分别为：晒晾烟〉黄花烟〉烤烟。3个群体间遗传距离比较分析,晒晾烟与烤烟的遗传相似度达到0.9840。（3）聚类分析可将78份种质材料划分为黄花烟草和普通烟草2大类群,烤烟、晒晾烟之间没有明确的界线。聚类分析结果也表明,黄花烟与其他群体的遗传距离较远。

Genetic variation within 78 tobacco germplasms resistance to PVY, including three populations of flue-cured, sun-cured, and N. rustica L. were investigated using SSR marker. The results were as followed that totally 108 loci were produced with 96 polymorphic loci（ 88.89% ）using 45 different primer combinations selected from 960 primers combinations. A high level of genetic differentiation among populations was detected based on Nei＇s genetic diversity analysis（0. 3766 ） , Shannon＇s information index analysis（ 0. 5821 ） , and the genetic similarity coefficient variation range （ 0. 0454 - 0. 9973 ）. The genetic differentiation was different with- in the three populations： sun-cured 〉 N. rustica L. 〉 flue-cured. Compared the genetic distances among three groups, the similarity of sun-cured and flue-cured tobacco was 0. 9840. The cluster analysis indicated 78 tobac- co germplasms were divided into N. rustica L. and N. tabacum L groups. There was not a clear boundary line between sun-cured and flue-cured. The genetic distance was far between N. rustica L. population and the other N. tabacum L. populations.