食管鳞状细胞癌组织中抑癌基因Dab2和细胞外信号调节激酶1表达的临床意义

Expressions of Dab2 and ERK1 in esophageal carcinoma tissues and their clinical significance

目的探讨食管鳞状细胞癌（简称鳞癌）组织中抑癌基因Dab2和细胞外信号调节激酶1（ERK1）表达的临床意义。方法采用免疫组化、原位杂交及反转录一PCR技术检测59例食管鳞癌组织、27例不典型增生组织和36例正常食管黏膜组织中Dab2和ERK1蛋白及mRNA的表达。结果食管鳞癌组织中Dab2蛋白及mRNA表达阳性率显著低于其他两种组织（23．7％比94．4％、44．4％及18．6％比97．2％、33．3％），而ERK1蛋白及mRNA表达阳性率显著高于其他两种组织（81．4％比44．4％、63．0％及78．0％比30．6％、51．9％）（均P〈0．05）。Dab2、ERK1蛋白及mRNA表达与淋巴结转移显著相关，且ERK1蛋白及mRNA表达与肿瘤浸润深度显著相关（均P〈0．05）。食管鳞癌组织中Dab2与ERK1的表达呈负相关（P〈0．05）。结论Dab2和ERK1与食管鳞癌的发生、发展和转移密切相关，其作用可能互为拮抗。

Objective To explore the expressions of protein and mRNA of disabled-2 （Dab2） and extracellular signal-regulated kinasel （ ERK1 ） in esophageal squamous cell carcinoma tissue （ ESCC ） and their clinical significance. Methods The expressions of protein and mRNA of Dab2 and ERK1 were detected in ESCC （ n = 59） , atypical hyperplasia （ n = 27 ） and normal esophageal mucosa （ n = 36 ） tissues by immunohistochemistry, in situ hybridization and reverse transcription polymerase chain reaction （RT- PCR）. Results The expressions of protein and mRNA of Dab2 were lesser in ESCC than those in atypical hyperplasia and normal esophageal mucosa tissues （23.7% vs 94.4% , 44. 4% and 18.6% vs 97.2% , 33.3% ）. And the expressions of protein and mRNA ERK1 were higher in ESCC than those in atypical hyperplasia and normal esophageal mucosa tissues （81.4% vs 44.4% , 63.0% and 78.0% vs 30. 6% , 51.9% ） （all P 〈 0.05）. Additionally, the expressions of protein and mRNA of Dab2 and ERK1 were closely related with lymph node metastasis in ESCC tissue and the expressions of protein and mRNA of ERK1 closely related with the depth of tumor invasion in ESCC tissue （ all P 〈 0. 05 ）. Furthermore, the results of correlation analysis revealed that expressions of protein and mRNA of Dab2 and ERK1 in ESCC had a negative correlation （ all P 〈 0. 05 ）. Conclusion The expressions of protein and mRNA of Dab2 and ERK1 play important roles in the infiltration, metastasis and carcinogenesis of ESCC and their effects may be antagonistic to each other.