红籽鸢尾高频离体再生条件的初步筛选

Preliminary selection of high frequency in vitro regeneration condition of Iris foetidissima

红籽鸢尾（Irisfoetidissima Linn．）为鸢尾科（Iridaceae）鸢尾属（Iris Linn．）多年生草本植物，原产于西欧和非洲北部。该种的叶鞘呈深绿色剑形，蒴果饱满且蒴果成熟开裂后露出红、橙黄和白等不同色泽的种子并一直垂挂到翌年春天，集观花、观叶和观果为一体，观赏价值较高。另外，该种还具有耐寒性好、在长江中下游地区四季常绿、适应性强及耐粗放管理等优点，是一种优良的常绿地被植物。

Taking stem tips of Irisfoetidissima Linn. as explants, effect of different phytohormone combinations on its callus and adventitious bud inductions and effect of different basic media and mass concentrations of NAA on rooting were studied, the high frequency in vitro regeneration condition of I. foetidissima was selected prehminarily. Results show that the optimum callus induction medium of I. foetidissima is MS medium containing 1.5 mg · L-1 2,4-D and 0.1 mg· L-1 6-BA, its callus induction rate is high to 77.27%. The optimum adventitious bud induction medium is MS medium added with 1.0 mg· L-t 6-BA and 0.2 mg· L-1 NAA, with adventitious bud induction rate of 72.22%. The optimum rooting medium is MS medium added with 0.2 mg · L-1 NAA, its rooting rate reaches to 100.00%. The survival rate of plantlets after transplanted is also high with a value of above 95%.