改良抗酸染色法在结核性脑膜炎诊断中的应用

Evaluation on a modified Ziehl-Neelsen stainin the diagnosis of tuberculous meningitis

目的探讨改良抗酸染色法在结核性脑膜炎诊断中的应用。方法采用改良抗酸染色法对35例结核性脑膜炎患者脑脊液进行涂片、染色及镜检。结果 35例患者中改良抗酸染色法阳性33例(94.29%),其中有53.40%的标本找到胞内抗酸杆菌;非结核性脑膜炎者15例,其中有1例改良抗酸染色结果阳性。结论改良的抗酸染色法显著提高了脑脊液抗酸杆菌的检出率,并能发现细胞内抗酸杆菌。因此,改良抗酸染色法可作为早期诊断结核性脑膜炎的有效检测手段。

Objective Toevaluatea modified Ziehl-Neelsen（Z-N） stain in the diagnosis of tuberculous meningitis. Methods Cerebrospinal fluid specimens from 35 patients were stained by using the modified Ziehl-Neelsen staining. Re- suits The positive rate was 94.29% in 35 patients with tuberculous meningitisand the intracellular acid-fast bacilli was detected in 53.40% of all specimens. One case was stained positive in 15 patients with non-tuberculous meningitis. Con- clusion The modified Ziehl-Neelsen stain not only significantly improves the detection rates of tuberculous meningitisbut alsois able to identify intracellular M.tuberculosisin cerebrospinal fluidspecimen.Thus, the modified Z-N stain can be a convenient tool for diagnosing tuberculous meningitis.