骨髓间充质干细胞抑制小胶质细胞介导的炎症反应

Bone marrow mesenchymal stem cells inhibit inflammatory response of microglial cells

目的研究骨髓间充质干细胞(Bone Marrow Mesenchymal Stem Cells,BMMSCs)对小胶质细胞介导的炎症反应的抑制作用。方法实验分为四组:组一:小胶质细胞(BV2)生长于DMEM(High Glucose)培养液中;组二:BV2细胞生长于加入脂多糖(LPS)的上述培养液中;组三:BV2细胞、BMMSCs共培养于加入LPS的上述培养液中;组四:骨髓间充质干细胞(BMMSCs)生长于加入LPS的上述培养液中。观察BV2细胞的生长状态、电镜超微结构变化及其分泌的炎症因子TNF-α表达量的变化。结果光镜下BV2细胞密度依次为:组一>组三>组二,组四中BMMSCs生长状态良好;电镜下可见组二BV2细胞内出现大量肿胀及空泡化的线粒体、内质网等细胞器,少见生长活跃多核仁细胞,同时可见大量崩解细胞,组三细胞状态明显好于组二;BV2细胞分泌的炎症因子TNF-α表达量依次为组二>组三>组一>组四。结论 BM-MSCs抑制小胶质细胞介导的炎症反应,进而发挥神经保护作用。

Objective To investigate the inhibitory effect of bone marrow mesenchymal stem cells （ BMMSC） on the inflammatory response of microglial cells .Methods The samples were divided into four groups .Group I：microglial （BV-2） cells were cultured in DMEM （high glucose）.Group II： BV-2 cells were cultured in DMEM containing lipopo-lysaccharide （LPS）.Group III：BV-2cells and BMMSCs were co-cultured in DMEM.Group IV： BMMSCs were cultured in DMEM containing LPS .The growth state and ultrastructure of BV-2 cells were observed and the changes of TNF-αex-pression were detected .Results Different cell densities of BV-2 cells were observed under the optical microscope in an or-der from high level to low level：group I 〉group III 〉group II.The expressions of TNF-αwere：group Ⅱ 〉groupⅢ〉group Ⅰ.Ultrastructural observation of BV-2 cells showed that there were a large number of swollen mitochondria and endoplasmic reticulum , some of them showed vacuolization .No BV-2 cells with multiple hucleoli were found in the group II indicating the absence of active cell growth .At the same time, cytolysis was observed only in the group II .The growth of BV-2 cells in the group III was better than that in the group II .Conclusions BMMSCs can inhibit inflammatory response＆amp;nbsp;of microglial cells, therefore, play a neuroprotective role.