高原低氧适应动物新疆灰旱獭右心室重构的组织学改变

Histological changes of the right ventricular remodeling in Xinjiang Marmota baibacina adapted to high-altitude hypoxia

目的探讨新疆灰旱獭高原低氧适应性改变致右心室重构组织学改变。方法应用免疫组化技术检测新疆灰旱獭右心室缝隙连接蛋白43(CX43)蛋白表达,同时应用HE染色和Masson染色观察心室肌结构和纤维化程度变化。结果心肌细胞肥大,胶原纤维增多,右心室肥厚指数、体重指数明显增高。CX43蛋白表达减少和(或)分布的改变。结论高原低氧致新疆灰旱獭右心室结构重构,可作为研究高原低氧适应性机制的理想动物模型。

Objective To investigate the histological changes of the right ventricular remodeling in Xinjiang Mar-mota baibacina which are adapted to high-altitude hypoxia .Methods Hematoxylin and eosin and Masson staining was used to examine the pathological changes and degree of fibrosis , and immunohistochemical technique was used to detect connexin 43 （ CX43） expression in the right ventricular myocardium .Results In the right ventricular myocardium , cardio-myocyte hypertrophy and increased amount of collagen fibers were observed .The right ventricular hypertrophy index and weight index of Marmota baibacina were significantly higher , and expression of CX43 was significantly reduced than that of normal rats （ P〈0.05 for both ） .Conclusions High-altitude hypoxia induces right ventricular remodeling in Xinjiang Marmota baibacina.It can be used as an ideal animal model for studies on the mechanisms of hypoxia adaptation on high -altitude plateau .