陆地棉不同组织器官原花青素含量分析

Assay of Procyanidin in Various Tissues and Organs of Gossypium hirsutum

旨在研究棉花不同组织器官中的原花青素的含量。采用可见分光光度法分别测定棉花品种‘中棉所35’的花萼、叶片、花瓣、茎和根等组织器官中原花青素的含量,比较其在各组织器官中的分布。分析结果表明,可溶性的原花青素在花萼中的含量最高,为0.040μg/mg(新鲜组织),叶片和花瓣中的含量较高,分别为0.030、0.017μg/mg,而根含量最低,为0.005μg/mg;非可溶性的原花青素含量在各组织器官中的含量与可溶性的分布呈大致相同的趋势,花萼、叶片、花瓣、茎和根含量分别为2.324、1.742、1.449、0.417、0.384μg/mg。在棉花中原花青素主要以非可溶性形式存在,而可溶性的含量较低,在花瓣中两者相差达到84倍,在茎里相差也达到29倍。研究结果暗示原花青素在各组织器官中分布不均衡可能与其执行不同功能有关,与棉花抗、耐逆性有着重要的关系。

The aim was to study the contents of procyanidin（PA） in different tissue and organs of cotton. The contents of PA in various tissues and organs of G. hirsutum cv. CRI35, including calyx, petal, leaf, stem and root, were analyzed by visible spectrophotometer. The results revealed that the contents of soluble PA in calyx was the highest, reaching to 0.040 μg/mg, while that in root was lowest, only 0.005 μg/mg. The contents in leaf and petal were moderate, respectively 0.030 and 0.017 μg/mg. And the contents of insoluble PA in various tissues and organs exhibited the similar content current as the soluble PA, which were respectively 2.324, 1.742, 1.449, 0.417 and 0.384 μg/mg correspondingly in calyx, leave, petal, stem and root. Insoluble PA accounted for major part in CRI35, while soluble PA was only minor part. In petal, insoluble PA content was 84 folds of soluble PA, while in stem, the rates of two kinds of PA content was smallest, reaching to 29 folds. Taken together, results suggested that the difference of PA in various tissues and organs of CRI35 could be related to their executing functions, especially, which helped exploit the resistance/tolerance of cotton in breeding.