尿激酶型纤溶酶原激活剂与血管内皮生长因子在胃癌中的表达及其意义

Expression and significance of urokinase-type plasminogen activator and vascular endothelial growth factor in gastric carcinoma

目的:探讨尿激酶型纤溶酶原激活剂(urokinase-type plasminogen activator,u-PA)以及血管内皮生长因子(vascular endothelial growth factor,VEGF)在胃癌中的表达和意义。方法:免疫组化法检测98例胃癌组织和30例癌旁正常组织中u-PA和VEGF的表达,并分析其相互关系。结果:胃癌组织中u-PA与VEGF的表达明显高于癌旁正常组织(P=0.000),u-PA与VEGF在低分化、浸润达浆膜层、有淋巴结转移及TNM分期(Ⅲ期+Ⅳ期)组阳性表达率均明显高于中、高分化、浸润未达浆膜层、无淋巴结转移及TNM分期(Ⅰ期+Ⅱ期)组(P<0.05)。u-PA与VEGF的表达呈显著正相关(rs=0.568,P=0.000)。结论:u-PA与VEGF在胃癌组织中均有较高的阳性表达率,并且与胃癌的侵袭转移相关,它们共同参与了胃癌的发生、发展,可作为判断胃癌侵袭转移潜能的重要分子生物学指标;同时,二者在胃癌的浸润转移过程中可能具有协同作用,联合检测u-PA和VEGF对胃癌的早期诊断、治疗和预后具有积极的指导作用。

Objective：To investigate the expression and significance of urokinase-type plasminogen activator（u-PA） and vascular endothelial growth factor（VEGF） in gastric carcinoma. Methods：Expressions of u-PA and VEGF was detected by immunohisto- chemistry in 98 gastric carcinoma specimens and 30 tumor-adjacent normal tissues. Relationship between u-PA and VEGF was analyzed. Results：Positive expression of u-PA and VEGF was significantly higher in gastric carcinoma than in tumor-adjacent normal tissues（P=0.000）. Expression of u-PA and VEGF in gastric carcinoma with low differentiation, serous membrane involvement, lymph node metastasis,TNM stage（m was significantly higher than that of u-PA and VEGF in gastric carcinoma moderate or high differentiation,no serous membrane involvement,no lymph node metastasis,TNM stage（ Ⅰ＋Ⅱ ）（p〈0.05）. Expression of u-PA was positively correlated with expression of VEGF（rs=0.568,P=0.000）. Conclusions ：u-PA and VEGF are highly expressed in gastric carcinoma tissues. They have correlation with invasion and metastasis of gastric carcinoma, and both of them are involved in the occurrence and development of gastric carcinoma,which can be used as potential molecular marker determining the invasion and metastasis of gastric carcinoma. Meanwhile, they may have synergistic effect on the invasion and metastasis of gastric carcinoma. Combined detection of u-PA and VEGF may play positive role in early diagnosis, treatment and prognosis of gastric carcinoma.