Has-miRNA-96在宫颈癌组织中的表达及其临床意义

Expression and clinical significances of Has-miRNA-96 in tissues of cervical cancer

目的：探讨Has-miRNA-96（miR-96）在宫颈癌组织中的表达情况及其与临床病理特征间的关系。方法： 采用茎环Real time RT-PCR方法检测52例宫颈癌、28例正常宫颈组织中Has-miRNA-96的表达，分析Has-miRNA-96表达与宫颈癌常见的临床病理特征的关系。结果：Has-miRNA-96在宫颈癌组织中的相对表达量（127.045±235.424）高于正常宫颈组织（0.995±0.236），差异有统计学意义（P=0.000）；在中、低分化癌的表达（137.778±249.981）高于高分化癌中的表达（75.766±147.237），差异有统计学意义（P=0.005）；在腺癌中相对表达量为（196.213±172.519），明显高于鳞癌（110.576±246.910），（P=0.004）；临床Ⅰ期、Ⅱ期、Ⅲ～Ⅳ期子宫颈癌患者组织中Has-miRNA-96表达水平分别为（22.614±25.828）、（122.493±78.043）、（672.902±476.169），Ⅲ～Ⅳ期高于Ⅰ期、Ⅱ期（P=0.000、P=0.001），Ⅱ期高于Ⅰ期，差异有统计学意义（P=0.000）；有淋巴结转移组表达为（142.537±104.673）明显高于无淋巴结转移组（19.787±26.204，P=0.000）；浸润深度：≥1/2间质表达为（75.323±94.822），高于＜1/2间质者（27.449±49.728，P=0.025）；而与患者年龄无明显关系（P=0.385）。结论：Has-miRNA-96在宫颈癌组织中存在异常高表达, 提示其可能在宫颈癌的发生发展中发挥致癌基因的作用，并且与宫颈癌的演进及不良预后密切相关。

Objective：To evaluate has-miRNA-96 （miR-96） expression in cervical cancer tissues and their association with the clinicopathological features. Methods ： Expression of miR-96 in 52 cases of cervical cancer tissues and 28 cases of normal tissues was examined by stem-loop real-time PCR. Correlations between expression of miR-96 and related clinicopathologic features of cervical cancer （age, pathologic pattern, histological grade, depths of interstitial infiltration, lymph node metastasis, clinical stage） were further analyzed. Results ：Relative expression of miR-96 was significantly higher in cancer tissues（127.045 ± 235.424） than in normal tissues（0.995 ± 0.236）（P=0.000）. Expression of miR-96 was significantly higher in middle and low differentiated carcinoma（ 137.778 ± 249.981 ） than in high differentiated carcinoma（75.766 ± 147.237）,with statistical differences （P=0.005）. Expression of miR-96 was significantly higher in cervical adenocarcinoma（196.213 ± 172.519） than in squamous cell carcinomas （110.576 ± 246.910）（P=0.004）. Expression of miR-96 at Ⅰ satge, Ⅱ stage and Ill-IV stage was （22.614 ± 25.828）, （122.493± 78.043） and （672.902 ± 476.169） respectively; expression at Ⅲ - Ⅳ stage was signifieantly higher than that at Ⅰ stage and Ⅱ stage （P=0.000,P=0.001） and expression at Ⅱ stage was significantly higher than that at Ⅰ stage（P=0.000）. Expression of miR-96 was significantly higher in those with lymphatic metastasis （ 142.537 ±104.673） than those without （ 19.787 ± 26.204） （P=0.000）. Patients with invasive depth 〈 1/2 interstitial substance（27.449 ± 49.728） showed lower level of miR-96 than those with invasive depth ≥ 1/2 interstitial substance（75.323 ± 94.822） （P=0.025）. Expression of miR-96 was not correlated with age （P=0.385）. Conclusions：Aberrant expression of miR-96 suggests it might play a role as an oncogene in the tumorigenesis and development of cervical carcinoma and it possibly correlates with progression and prognosis of cervical cancer.