姬松茸液体发酵菌丝体多糖的中试生产

Pilot scale production of crude polysaccharide from liquid fermentation mycelia of Agaricus blazei

通过中试试验确定了姬松茸液体发酵生产菌丝体的最佳条件和菌丝体多糖生产工艺。发酵工艺中碳源为玉米粉和蔗糖,氮源为豆饼粉和酵母粉,接种量5%,发酵温度25℃,通气量1∶0.5(v/v),搅拌速度150 r/min,发酵培养72 h,菌丝体平均得率16.42%,菌丝体中多糖含量为25.22%。液体发酵姬松茸菌丝体多糖的生产工艺为:菌丝体加10倍量水,100℃热回流2次(提取时间分别为2 h和1 h),合并2次提取滤液,用截留值为10 kD的中空纤维超滤柱将提取液超滤浓缩至浓缩比为1∶1,将浓缩滤液加2%麦芽糊精,喷雾干燥后得粗多糖,粗多糖得率为12.64%,粗多糖含量为33.47%。

The optimal conditions of producing Agaricus blazei mycelia through liquid state fermentation and the process of extracting polysaccharide from the mycelia were determined by pilot tests. Maize meal and sucrose were used as carbon sources, and soybean meal and yeast powder as nitrogen sources in fermentation, the mycelia yield was averagely 16.42%, and the polysaccharide content of mycelia was 25.22% under the conditions：inoculum concentration at 5%,ventilation ratio at 1 ： 0. 5 （v/v）, stirring speed at 150 r/min, and fermentation at 25 ℃ for 72 h. The process of producing polysaccharide of fermentation mycelia was as follows： the mycelia were added with ten fold distilled water, the extraction was made at 100 ~C firstly for 2 h and then for l h, the mixed filtrate was concentrated by 10-kD hollow fiber membrane ultrafiltration（concentration ratio of liquid to mycelia reaching 1：1）, the concentrated filtrate was added with 2% maltodextrin,and after spray drying the crude polysaccharide yield was 12.64% and its polysaccharide content was 33.47%.