硫氧还蛋白f介导的烟草Rubisco活化酶的氧化还原调控

Redox regulation of Tobacco Rubisco activase mediated by thioredoxin-f

利用菠菜和烟草的硫氧还蛋白f(Trx-f)在不同氧化还原状态下测定了菠菜、拟南芥、烟草和番茄Rubisco活化酶(RCA)活化其Rubisco的活力,实验结果显示菠菜Trx-f只能介导调控菠菜和拟南芥RCA的氧化还原状态,从而改变其活力,而烟草Trx-f介导也只能改变烟草和番茄RCA的氧化还原状态.上述结果表明烟草等物种的单亚型RCA也能受Trx-f介导的氧化还原调控,而且Trx-f介导的RCA氧化还原状态的改变具有种属专一性.

We measured the activation activities of RCA from spinach,arabidopsis,tobacco and tomato under different redox states in the presence of Trx-f from spinach and tobacco,respectively. The results showed that the activity of RCA from spinach and Arabidopsis was regulated by different redox states in the presence of Trx-f from spinach,and the activity of RCA from tobacco and tomato was regulated by different redox states in the presence of Trx-f from tobacco. It demonstrated that that both the activity of RCA consisted of two isoforms and only one isoform（like tobacco） can be regulated by changes in the redox states,which mediated by the Trx-f. Furthermore,we also suggested changes in the redox state of RCA mediated by Trx-f are species dependent.