摘要
为探讨乳苣水提取物对体外培养的肺癌细胞H1299和A549的影响,用不同浓度的乳苣水提取物分别作用于H1299和A549细胞,从细胞形态(显微镜观察)、细胞增殖活力(cck8法)、细胞凋亡(AnexinⅤ-FITC/PI染色)、线粒体膜电位(JC-1染色)几个方面检测乳苣水提取物对H1299和A549细胞的影响.当处理浓度达到1.2 mg/mL,作用细胞48 h后,细胞出现明显皱缩的凋亡形态学特征,与对照组相比处理组细胞增殖活力显著下降(P<0.05).进一步用流式细胞仪检测发现浓度达到1.2 mg/mL的处理组细胞凋亡率显著提高,同时检测到细胞的膜电位都明显下降,呈剂量依赖关系.这些结果表明乳苣可以通过诱导H1299和A549细胞凋亡从而抑制其生长和增殖,是一种潜在的预防和抑制肿瘤生长的药食两用植物.
To illustrate the effects in human lung cancer H1299 and A549 cells treated with Mulgedium tataricum(Linn.) DC.(MTDC) aqueous extracts,assays were performed. These assays included morphologic changes observed by microscope,cell proliferation vitality tested by cell growth assay using cck8 kit,the apoptosis detected by Flow Cytometry,mitochondrial membrane potential assessed with the fluorescent probe JC-1. Compared with the control cells,cells treated with high dose( ≥1. 2 mg / mL) of MTDC were shrunken and their morphological characteristics of apoptosis was obvious under microscope,cell proliferation was significantly inhibited(P 〈 0. 05). Furthermore,the apoptosis cells were significantly increased contrast with the control group revealed by flow cytometry analysis and the significant decrease of cell mitochondrial membrane potential showed a dose-dependent effect. The data showed that MTDC may inhibit the growth and proliferation of human lung cancer H1299 and A549 cells through inducing cell apoptosis. MTDC is a potential edible herb for cancer prevention and treatment.
出处
《上海师范大学学报(自然科学版)》
2014年第2期196-203,共8页
Journal of Shanghai Normal University(Natural Sciences)
基金
上海市大学生创新活动计划项目
关键词
乳苣水提取物
肺癌细胞
增殖
凋亡
Mulgedium tataricum(Linn.) DC.
lung cancer cell
apoptosis
proliferation