摘要
采用组织分离法,对采自广西岑溪市的普通油茶(Camellia oleifera)和广西宁明县的陆川油茶(Camellia vietnamensis)叶片和果实中的油茶炭疽菌进行分离,共得到4株油茶炭疽菌。对4株纯化菌株进行了形态学观察和基于rDNA-ITS序列的分子系统发育树分析。结果表明分离自油茶叶片的菌株742、GW2、CR1聚为1组,分离自果实的菌株CRF1单独聚为1组,分离自油茶的4株炭疽菌菌株属于胶孢炭疽菌(Colletotrichum gloeosporioides)复合群的种类,有可能为尚未发表的2个新的分类单元。
The Colletotrichum sampled from Camellia oleifera in Cenxi City and Camellia vietnamensis in Ningming city,Guangxi,were isolated via tissue methods and 4 isolates of Colletotrichum were obtained .Mor-phological description and phylogenetic analysis were made based on rDNA ITS sequnces .The results showed that the strains of 742,GW2 and CR1 isolated from leaves were clustered into one group ,and the CRF1 isola-ted from fruit was clustered into another group ,respectively .All the 4 strains belonged to Colletotrichum gloeos-porioides species complex .
出处
《江西农业大学学报》
CAS
CSCD
北大核心
2014年第2期314-318,共5页
Acta Agriculturae Universitatis Jiangxiensis
基金
广西科学研究与技术开发计划项目(桂科攻1123004-2A
桂科能1298022-3-2)
广西自然科学基金项目(2011GXNSFB018037)
关键词
油茶
炭疽病
病原鉴定
Camellia oleifera
Colletotrichum
pathogen identification
