摘要
以多糖得率为指标,水料比、提取时间、提取温度为考察因素,在单因素试验的基础上,采用响应面法对核桃(Juglans regia L.)青皮多糖超声辅助提取工艺进行优化,并对核桃青皮多糖清除羟基自由基和超氧阴离子自由基活性进行了分析。结果表明,核桃青皮多糖超声辅助提取最佳工艺条件为:水料比18∶1(V∶m)、提取时间31 min、提取温度80℃、提取2次,在此条件下多糖得率为9.02%;核桃青皮多糖对羟基自由基及超氧阴离子自由基均有一定的清除活性,但其清除作用小于维生素C。
The factors affecting the extraction of polysaccharides from walnut（Juglans regia L.） green husk were investigated. Based on the results of single-factors test,the Response surface methodology（RSM） was used to optimize the extraction of polysaccharides from walnut green husk. Antioxidant activities of poly-saccharides were evaluated by scavenging capability of hydroxyl free radicals and superoxide anion radicals. The results showed that the optimum conditions were the ratio of water to material of 18∶1（V∶m）, extraction time of 31 min, extraction temperature of 80 ℃, extract 2 times. Under these conditions, the yield of polysaccharides was 9.02%. The polysaccharides of walnut green husk have certain antioxidant activities. The scavenging activity for hydroxyl free radicals and superoxide anion radicals was lower than that of vitamin C.
出处
《湖北农业科学》
北大核心
2014年第5期1148-1152,共5页
Hubei Agricultural Sciences
基金
山西省化学优势重点学科建设项目(912019)
