聚合酶链式反应实验教学的组织与效果分析

Experimental Teaching Example of Polymerase Chain Reaction

聚合酶链式反应是常规分子生物学实验的重要内容之一。作者在多年从事分子生物学实验教学的基础上,根据聚合酶链式反应实验特点,在实验操作的间隔时段引入相关问题的探讨。整个教学过程由学生查阅资料进行问题和实验准备、教师对实验原理与操作注意事项的简单介绍、实验操作、问题讨论、实验结果观察与分析等组成。实施效果表明,作者采用的教学组织方案能够更加有效地利用实验过程中的间歇时间,通过对预先设定的问题和学生自主提问进行探讨,实现良好的师生互动,加深了学生对聚合酶链式反应实验原理及相关理论的理解与掌握,增强学生利用所学理论知识分析实际问题的能力。对于激发学生的学习积极性具有较好的促进作用。

Polymerase chain reaction is a powerful tool for in vitro amplication of specic nucleic acid sequences and has been assigned one of the important content of the conventional molecular biology experiments as its wide usage in life science related subjects and its impact on the scientic community involving in basic research and practical analysis.The author provide a practicable teaching scheme focusing on the quality-oriented education within which problem discussion was properly introduced into the course during running polymerase chain reaction and/or the quench time of electrophoresis.The whole process of this experiment includes problem preparation,teacher＇s explanation,students＇ operation,problem discussion,observation and analysis of the experiment result.Teaching practice proved that this scheme can effectively use the interval time,through problem discussion,to stimulate the interaction and communication between teachers and students,help students of to understand the principle and related theoretical aspects of polymerase chain reaction,enhance the students＇ ability of using theoretical knowledge to analysis the actual problem.Learning interest can be also promoted.