大鼠实验性牙周炎模型的快速建立

Rapid establishment of periodontitis model in rats

目的:探讨如何快速建立大鼠实验性牙周炎模型,为开展牙周病的病因及防治的研究提供简便可靠的造模方法。方法:20只5周龄SD大鼠,采取"牙龈划割+钢丝结扎+牙周炎患者细菌培养物冲洗+高糖饮食"的办法进行造模,在第2周末和第4周末观察牙龈炎症、牙周袋深度及牙松动度、牙槽骨吸收情况等指标,确认是否成功建模。结果:在第4周末实验牙牙槽骨吸收已深达根尖,其余指标也均显示成功建立了大鼠重度牙周炎模型。结论:使用该方法能够比较快速简便的构建大鼠重度牙周炎模型,从而有效模拟临床牙周炎发展进程,为牙周病的病因研究及临床防治提供依据。

Objective： To establish an experimental rodent model of periodontitis for the understanding of the progression, prevention,and treatment of the disease. Methods： 20 five-week old rats received a gingival incision followed by steel wire suture, then inoculated with periodontitis bacterial suspension and fed with a high-glucose diet.Several indexes were recorded on the second and fourth week. Resuits： The alveolar bone loss reached the apex of the root by the end of the fourth week. Other indexes also indicated that a severe periodontitis was established. Conclusion：We have successfully established a simple but reliable method to imitate severe periodontitis in rats. This method can be used to better understand the progression of periodontitis and provide a basis for further study on etiology, prevention and treatment of periodontitis.