基于ISSR标记的杉木种源遗传多样性分析

Genetic Diversity of Chinese Fir Provenances Using ISSR Markers

为从分子水平探讨杉木（Cunninghamia lanceolata）种源空间遗传变异模式,采用ISSR分子标记对杉木全分布区内的40个种源进行遗传多样性分析。结果显示：9条ISSR引物共检测出133条带,其中122条多态性条带,多态条带百分率（PPB）为91.73%,各种源PPB和Shannon表型多样性指数（HPOP）分别为37.46%～55.75%和0.201 5～0.344 5,物种水平多态性条带百分率和Shannon信息指数（HSP）分别为89.86%和0.565 5;分子方差分析（AMOVA）揭示,种源间遗传分化系数（ΦST）为0.4651,这表明杉木种源具有较高的遗传多样性,种源间遗传分化较大。UPGMA法聚类表明,参试的40个杉木种源可分为7地理种源区。

In order to discuss the spatial genetic variation model of Chinese fir provenances from molecular level,the genetic diversity of 40 Chinese fir（ Cunninghamia lanceolata） provenances was assessed using Inter- simple sequence repeat（ ISSR） markers. Results showed that nine ISSR primers were generated 133 DNA bands and 122 DNA bands were polymorphic with PPB 91. 73%. The PPB within provenances ranged from 37. 46% to 55. 75%,and the Shannon＇s phenotypic diversity index（ HPOP） within provenances ranged from 0. 201 5 to 0. 344 5. The percentage of polymorphic bands and the Shannon information index（ Hsp） were 89. 86% and 0. 565 5 respectively. Analysis of molecular variance（ AMOVA） revealed that the genetic differentiation coefficient（ ΦST） of 40 provenances was 0. 465 1. It indicated that the different provenances of Chinese fir had higher genetic diversity. The 40 provenances were divided into seven major geographical provenance regions based on the UPGMA cluster analysis.