大电导钙激活钾通道β1亚基与子痫前期的相关性研究

Correlation Study of Large Conductance Ca^(2+)-Activated K^+-Channel β1 Subunit Interacts with Preeclampsia

目的:探讨胎盘组织和子宫平滑肌组织中大电导钙激活钾通道β1亚基(KCNMβ1)的表达,及其与子痫前期发病的关系。方法:轻、重度子痫前期组(研究组)和正常孕妇组(对照组)孕妇的胎盘组织及子宫平滑肌组织中KCNMβl mRNA的表达通过荧光定量PCR技术检测。采用ELISA法检测三组孕妇血清KCNMβl浓度变化水平。结果:各研究组中孕妇血清的KCNMβl水平、胎盘组织及子宫平滑肌中KCNMβl mRNA表达水平均明显低于对照组,差异均有统计学意义(P<0.05);而轻、重度子痫前期组比较差异均无统计学意义(P>0.05)。结论:孕妇血清中KCNMβl表达降低,胎盘组织和子宫平滑肌中KCNMβl mRNA的表达降低与子痫前期的发病密切相关,可能参与了子痫前期的病理生理过程。

Objective： To investigate the relationship between the pathogenesis Of preeclampsia （ PE ） and the mRNA and protein expression of KCNM β 1 subunit in placenta and uterine smooth muscle tissue.Method： The pregnant placenta tissue and KCNM β1 mRNA expression in uterine smooth muscle tissue of the mild and severe preeclampsia group （ the study group ） and the normal pregnant women （ the control group ） were detected by fluorescent quantitative PCR technique. The serum KCNM β1 concentration level changes of three groups pregnant women were detected by ELISA method. Result： The pregnant women＇ serum level of KCNM β1, placenta and uterus smooth muscle in KCNM β1 mRNA expression levels of the research group were significantly lower than the control group, the difference had statistical significance （ P〈0.05 ） ; there were no significant difference between the mild and severe preeclampsia group （ P〉0.05 ） . Conclusion： KCNM β1 expression in serum of pregnant women is reduced, the placenta tissue and smooth muscle of the uterus KCNM β1 mRNA expression in lower closely associated with the onset of preeclampsia, may be involved in the pathophysiological process of preeclampsia.