紫癜性肾炎患者血清金属蛋白酶组织抑制物-1水平变化和临床意义

The Detection Changes and Clinical Significance of Tissue Inhibitors of Metalloproteinase-1 in Patients with Henoch Schonlein Purpura Nephritis

目的：观察不同分期过敏性紫癜性肾炎（Henoch-Schonlein purpura nephritis,HSPN）患者和健康人血清中金属蛋白酶组织抑制物-1（tissue inhibitors of metalloproteinase-1,TIMP-1）水平的变化,初步探讨TIMP-1在HSPN不同阶段中的作用及其与肾损害程度的关系,通过相关药物阻断TIMP-1的表达或抑制其活性,可能为预防、治疗肾脏纤维化提供一个新方法,以期达到理想治疗效果。方法：将过敏性紫癜性肾炎患者42例（试验组）按病理类型分组,并设立健康对照组。抽取两组的外周静脉血5 mL,分离血清,应用ELISA双抗体夹心法分别对其进行检测,观察各组TIMP-1水平的表达情况。结果：试验组Ⅱ～Ⅵ级组血清中TIMP-1水平较健康对照组显著增高（P〈0.05）,Ⅲ～Ⅵ级组血清中TIMP-1水平较Ⅱ级组显著增高（P〈0.01）,Ⅰ级组血清中TIMP-1水平与健康对照组比较差异无统计学意义（P＆gt;0.05）。结论：通过检测TIMP-1水平,再结合其他化验指标和临床表现,有助于对该疾病的治疗效果或者预后给予判断,对减少肾功能衰竭发生率具有指导意义。

Objective： To study the changes of serum tissue inhibitors of metalloproteinase-1 （ TIMP-1 ） in different stages of patients with Henoch-Schonlein pm＇pura nephritis （ HSPN ）, to discuss the role of TIMP - 1 in HSPN and its relationship with renal damage. By blocking the expression of TIMP - 1 or inhibit its activity, to provide a new method for the treatment of renal fibrosis. Method： 42 patients with HSPN （ experimental group ） were divided into six groups according to the pathological types, and normal control group was set up. 5 mL peripheral vein blood from all of the research object were extracted and separated its serum. Double antibody sandwich ELISA method was used to detect the expression of IMP-1 in each group. Result： The levels of serum TIMP-1 in Ⅱ - VI grade HSPN were significantly higher than those in the normal control group （ P〈0.05 ）, and its levels in Ⅲ - IV grade HSPN were significantly higher than those in the Ⅱ grade （P〈0.01） . But compared with the normal control group, the levels of TIMP-1 in I grade HSPN had no statistical significance （ P〉O.05 ） . Conclusion： By detecting TIMP - 1 level, in combination with other test indicators and clinical symptoms, can help the treatment of the disease or to judge prognosis, it has the instruction significance to reduce the incidence of renal failure.