维生素A纳米微乳的吸收动力学研究

Study on Absorption Kinetics of Vitamin A Nano-emulsion

以海赛克斯蛋鸡为试验动物,采用高效液相色谱法（HPLC）检测血清视黄醇在口服不同剂型维生素A（VA）后的动态变化。所得数据用PKSolver 2.0软件进行药代动力学相关参数分析,结果表明：脂溶型VA的血药峰浓度（Cmax）为（0.29±0.02）μmol/mL,达峰时间（Tmax）为（2.47±0.10）h,药时曲线下面积（AUC0～t）为（1.44±0.24）μmol/mL·h,吸收半衰期（t1/2α）为（1.68±0.01）h；水溶型VA的Tmax和t1/2α分别为（1.44±0.15）h和（0.92±0.01）h,均显著低于脂溶型VA（P〈0.05）,其余各参数与脂溶型VA相比无显著差异；纳米型VA的Tmax和t1/2α分别为（1.19±0.02）h和（0.83±0.03）h,均极显著低于脂溶型VA（P〈0.01）,纳米型VA的Cmax为（0.38±0.004）μmol/mL,极显著高于脂溶型VA（P〈0.01）,AUC0～t为（1.99±0.29）μmol/mL·h,显著高于脂溶型VA（P〈0.05）。以脂溶型VA为参比剂型,纳米微乳型VA的相对生物利用度为138.19%（P〈0.05）。综上,纳米微乳型VA在吸收速率、吸收峰值以及生物利用度方面优于脂溶型VA。

Dynamic change of the retinol from plasma samples of Hexics Brown hens were detected by HPLCafter oral administration with different forms of vitamin A,including lipo-soluble（lsVA）,water-soluble（wsVA）andnano-emulsion vitamin A（neVA）. The data were analyzed to obtain the pharmacokinetics parameters via the software ofPKSolver2.0.The results showed as following：as for lsVA,the peak concentration（Cmax）was（0.29±0.002）μmol/mL,timeto peak（Tmax） was（2.47±0.10）h,the area under the concentration-time curve（AUC0～t）was（1.44±0.24）μmol/mL·hand the absorption half life time（t1/2α）was（1.68±0.01）h. While the Tmax and t1/2αof wsVAwere（1.44±0.15）h and（0.92±0.01）h,respectively,which were significantly lower than those of lsVA. The Tmax and t1/2αof neVAwere（1.19±0.02）h and（0.83±0.03）h,respectively,which were significantly lower than those of lsVA,while the Cmax was（0.38±0.004）μmol/mL,which was significantly higher than that of lsVA.The AUC0～tof neVAwas（1.99±0.29）μmol/mL·h,which was significantly higher than that of lsVA. Compared with lsVA,the relative bioavailability of neVAwas 138.19%（P0.05）. To sum up,neVAwas superior to lsVAin absorption rate,peak absorption and bioavailability.