MTNR1a基因在内蒙古绒山羊下丘脑、垂体及卵巢中的表达

Expression of MTNR1a gene in hypothalamus, pituitary and ovary of Inner Mongolian cashmere goats

采用RT-PCR和原位杂交方法检测MTNR1a mRNA在内蒙古绒山羊下丘脑、垂体及卵巢中的表达及分布。结果表明,MTNR1a基因在内蒙古绒山羊下丘脑、垂体、卵巢中均有表达,且在下丘脑、垂体组织中呈广泛表达,在卵巢的卵泡膜细胞阳性表达。下丘脑、垂体、卵巢是褪黑激素作用靶器官,阐明褪黑激素对内蒙古绒山羊繁殖力的影响是通过与靶器官受体基因结合发挥其功能,为进一步探讨褪黑激素对内蒙古绒山羊繁殖影响的作用途径及调控机理奠定基础。

Using RT-PCR and in situ hybridization method to check MTNR1a mRNA expression localization in hypothalamus, pituitary and ovary of Inner Mongolian cashmere goats. It turned out that MTNR1a expressed in hypothalamus, pituitary and ovary of Inner Mongolian cashmere goats, in addition, there were a stronger expression signal in the hypothalamus and pituitary, the expression signal could be seen in thecal cells of ovary. Therefore, hypothalamus, ovary and pituitary were the target organs of melatonin. It also showed melatonin, which combined with target organs＇ receptor gene, affected reproductive performance of Inner Mongolian cashmere goats, which laid a foundation for making a further explore to the pathway, regulation and control mechanism with melatonin.