摘要
目的:高效液相色谱-质谱联用法(HPLC-MS)分析木瓜中绿原酸与抗癌活性成分的相关性。方法:采用HPLC-MS联用法,在Diamonsil C18色谱柱(4.6 mm×250 mm,5μm),乙腈-0.05%磷酸水溶液梯度洗脱;流速为0.8 ml/min;检测波长为325 nm;柱温为25℃。分离测定木瓜中绿原酸的含量。结果:木瓜中绿原酸的含量为1.3 mg/g,相对标准偏差(RSD)为0.4%,线性关系良好。结论:木瓜中含有绿原酸,其主要成分与木瓜对脂肪酸合酶(FAS)的抑制及抗肿瘤作用有潜在的相关性。
Objective： To analyze chlorogenic acid of Chaenomeles speciosa by high performance liquid chromatography-mass spectrometry. Methods： A method for determining chlorogenic acid of Chaenomeles speciosa by HPLC-MS is described. The operating conditions were Diamonsil C18column（5 μm, 4.6×250 mm） at room temperature, acetonitrile-0.05% phosphoric acid solution as mobile phase by step gradients elution at a flow of 0.8 ml/min and UV detection at 325 nm.Results： The content of chlorogenic acid of Chaenomeles speciosa is 1.3 mg/g（RSD 0.4%）,respectively. Conclusion： Chlorogenic acid was detected from Chaenomeles speciosa, which may be related to its inhibition of FAS and the potential role of anticancer.
出处
《中国医学装备》
2014年第5期4-6,共3页
China Medical Equipment
基金
国家自然科学基金(30500648)"以FAS为靶从元宝枫发展无耐药性的抗肿瘤药物"
北京市自然科学基金资助(7103168)"以介孔材料为载体从天然产物发展抗肿瘤靶向给药系统"
北京市教育委员会科技发展计划面上项目(KM201010025011)"从虎杖等天然产物中发展肿瘤化学预防药物"
首都中医药研究专项(ZM02)"医院制剂抗瘤丸治疗神经胶质瘤的作用机理研究"
