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法舒地尔对星形胶质细胞炎性分泌的影响

Effect of Fasudil on Inflammatory Secretion of Astrocytes
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摘要 目的观察法舒地尔对星型胶质细胞分泌炎性因子TNF-α和IL-8的影响。方法原代培养SD大鼠脑星形胶质细胞,将其随机分为对照组、糖氧剥夺组(OGD组)、法舒地尔干预组3组,在培养0、3、6、12h和24h收取上层培养液,用ELISA法测定各组培养液中TNF-α和IL-8的浓度。结果与对照组相比,OGD组星形胶质细胞分泌的TNF-α和IL-8在观察时间点内明显升高(均P<0.05),法舒地尔干预组星形胶质细胞分泌的TNF-α和IL-8也有升高,但较OGD组明显降低(均P<0.05)。结论 OGD可以刺激星形胶质细胞分泌TNF-α和IL-8,而法舒地尔能有效抑制OGD星形胶质细胞对TNF-α和IL-8的分泌,提示法舒地尔在星形胶质细胞分泌TNF-α和IL-8的调控中发挥重要作用。 Objective To observe the effect of Fasudil on inflammatory factors（TNF-αand IL-8）secreted by astrocytes.Methods Primary rat spinal astrocytes were cultured and randomly divided into three groups：control group,oxygen-glucose deprivation group（OGD group）and Fasudil group.The culture media were harvested at different time points（0,3,6,12, 24h）for detection of the concentration of TNF-αand IL-8by ELISA.Results Compared with the control group,the concentrations of TNF-αand IL-8in OGD group were significantly increased at each time point（P〈0.05）;in Fasudil group,the concentrations of TNF-αand IL-8increased at some time points,but they were much lower than in OGD group（P〈0.05）.Conclusion OGD can promote the secretion of TNF-αand IL-8by astrocytes in vitro,which can be effectively inhibited by Fasudil.It is suggested that Fasudil plays an important role in regulating the levels of TNF-αand IL-8secreted by astrocytes.
作者 段智慧 马聪敏
机构地区 郑州大学附属洛阳中心医院神经内科
出处 《华中科技大学学报（医学版）》 CAS CSCD 北大核心 2014年第2期219-222,共4页 Acta Medicinae Universitatis Scientiae et Technologiae Huazhong
关键词 法舒地尔 星形胶质细胞 肿瘤坏死因子-Α 白细胞介素-8 Fasudil astrocyte TNF-α IL-8
分类号 R741 [医药卫生—神经病学与精神病学]
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华中科技大学学报（医学版）
2014年 第2期

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