诱导多能干细胞治疗大鼠糖尿病足溃疡的效果研究

Effect of Induced Pluripotent Stem Cell in Treatment of Diabetic Foot Ulcers in Rats

目的观察局部移植异体诱导多能干细胞(induced pluripotent stem cell,IPSC)治疗糖尿病足溃疡SD大鼠的效果及其对外周血血管内皮生长因子(vascular endot growth factor,VEGF)的影响。方法取SD雄性大鼠40只,随机分为IPSC治疗组和对照组,分别对两组造糖尿病足溃疡模型。造模成功后IPSC治疗组在溃疡创面创缘肉膜层多点注射IPSC悬浊液,对照组在溃疡创面创缘肉膜层多点注射等体积Knockout DMEM培养液。观察比较两组治疗第1、5、10天溃疡面积及外周血VEGF水平。结果 40只SD雄性大鼠均成功造模。两组溃疡面积比较,治疗第1天差异无统计学意义(P>0.05);治疗第5、10天IPSC治疗组溃疡面积均小于对照组,差异有统计学意义(P<0.05)。两组外周血VEGF水平比较,治疗第1天差异无统计学意义(P>0.05);治疗第5、10天IPSC治疗组外周血VEGF水平均高于对照组,差异有统计学意义(P<0.05)。结论异体移植IPSC可促进糖尿病足溃疡SD大鼠溃疡愈合及局部新生血管形成。

Objective To observe the curative effect of allogeneic induced pluripotent stem cells（ IPSC）in treatment of diabetic foot ulcers in SD rats by local transplantation,and the effect on vascular endothelial growth factor（ VEGF）. Meth-ods A total of 40 male SD rats were randomly divided into IPSC treatment group（n=20）and control group（n=20）. Rat＆amp;nbsp;models of diabetic foot ulcers were established in the two groups. In the IPSC treatment group,ulcer wounds were multipointly injected with IPSC suspensions into tunica dartos retia,while the control group was injected with the same volume of Knockout DMEM culture solution. The ulcer areas on the 1st d,5th d and 10th d of treatment and VEGF levels in peripheral blood of the two groups were observed and comparedResults The 40 SD rat models were successfully established. There was no significant difference in ulcer area on the 1st d of treatment in the two groups（P﹥0. 05）;ulcer areas in IPSC treatment group on the 5th d and 10th d of treatment were significantly smaller compared with those in control group（P﹤0. 05）. There was no significant difference in VEGF level of peripheral blood on the 1st d of treatment in the two groups（P﹥0. 05）;levels of peripheral blood in IPSC treatment group on the 5th d and 10th d of treatment were significantly higher than those in control group（P﹤ 0. 05）. Conclusion IPSC allotransplantation may promote the healing of diabetic foot ulcers and local neovascularization in SD rats.