离子色谱法测定烟用香精香料中的硝酸根和亚硝酸根

Determination of nitrate and nitrite in tobacco casing and flavors by ion chromatography

对烟用香精香料中的NO3–和NO2–采用振荡萃取,建立了快速检测烟用香精香料中的NO3–和NO2–的离子色谱法（IC）。香精香料试样中的NO3–和NO2–在振荡条件下用水、二氯甲烷萃取,经0.22μm滤膜净化,柱流速为1.0 mL/min,采用浓度梯度洗脱方式,IonPac AS11阴离子分析柱、电导检测器检测,并采用该方法测定了15个烟用香精香料样品。结果表明：①NO3–和NO2–的检出限、回收率、相对标准偏差（RSD）及线性范围分别为0.010和0.006μg/mL,95.4%和90.6%,3.42%和4.61%,0.06～6.0μg/mL和0.02～2.0μg/mL;②测定的15个烟用香精香料样品中NO3–的检出率53.3%,NO2–的检出率40.0%。该方法具有快速、灵敏、简便等优点,适合于烟用香精香料样品中NO3–和NO2–的测定。

A method for simultaneously determining NO3- and NO2- in tobacco casing and flavors was developed based on ion chromatography（IC） and oscillation extraction. NO3- and NO2 – in casing and flavors were dealt with oscillation extraction by utilizing water and dichloromethane. They were purified through membrane（ 0.22 μm） with column flow rate of 1 mL /min.and radient elution was performed. The samples were separated on an Ion Pac AS 11 column and detected with an electrical conductivity detector. Results showed that： ① Limits of detection of NO3- and NO2- were between 0.010 and 0.006 μg/mL,with recovery rate ranging from 90.6% to 95.4% and RSDs from3.42% to 4.61%, and linear range of 0.06 to 6.0 μg/mL and 0.02 to 2.0 μg/mL,respectively. ② NO3- and NO2- in 15 tobacco casing and flavor samples were detected with detection rate of 53.3% and 40.0%. The method was proved to be applicable in determining NO3- and NO2- in tobacco casing and flavors as timesaving, accurate and simple to process.