耐甲氧西林金黄色葡萄球菌快速分型方法的研究

A study of rapid genotyping method for methicillin-resistant Staphylococcus aureus

目的基于聚合酶链反应(PCR)-高分辨率熔解(HRM)曲线分析方法和葡萄球菌蛋白A(SPA)分型方法,建立一种适用于耐甲氧西林金黄色葡萄球菌(MRSA)的快速分型方法。方法收集临床分离的71株MRSA作为试验菌株,采用基因测序和HRM曲线分析方法对菌株进行SPA基因分型。结果采用基因测序方法,71株MRSA菌株的SPA基因分为4个型别,分别是t570、t030、t002和t588,以t570(53例)为主要型别,其次是t030和t002(均为7例)。HRM分析对SPA基因的分型结果与基因测序方法的结果基本一致。结论 PCR-HRM分析有望成为一种快速、有效的MRSA的SPA基因分型方法,为医院感染控制提供依据。

Objective To establish a rapid genotyping method of for methicillin-resistant Staphylococcus aureus（MRSA） based on polymerase chain reaction（PCR）-high resolution melting （HRM ） curve analysis and staphylococcal protein A （SPA ） classifica-tion .Methods 71 strains of MRSA clinically isolated were collected as test strains .Gene sequencing and HRM curve analysis were employed to conduct SPA gene typing .Results According to gene sequencing method ,SPA gene of 71 strains of MRSA was divided into four types ,namely t570 ,t030 ,t002 and t588 .The most predominant type was t570 （74 .65% ） ,followed by t030 and t002（both 7 cases） .The result of SPA gene typing by HRM analysis were basically consistent with that by gene sequencing .Con-clusion PCR-HRM analysis is expected to become a fast ,efficient genotyping for MRSA SPA gene ,providing the basis for hospital infection control .