肛门、直肠常见病变中HPV感染的研究

Study of HPV infection in common anus and rectal disease

目的探讨肛门、直肠常见病变组织中人乳头状瘤病毒(HPV)感染的情况。方法采用基因扩增结合基因芯片技术对566例肛门、直肠常见病变组织标本进行HPV基因分型检测。结果 566例肛门、直肠常见病变组织中,总HPV感染率为32.86%(186/566);男性总HPV感染率、一重感染率和多重感染率分别为32.14%(117/364)、23.35%(85/364)及8.79%(32/364),与女性[分别为34.16%(69/202)、24.75%(50/202)及9.41%(19/202)]比较,差异无统计学意义(P>0.05),HPV 18、16、33、31型是肛门、直肠常见病变中的主要型别。结论肛门、直肠组织HPV基因分型检测对肛门、直肠HPV感染的分子流行病学研究具有重要意义。

Objective To study the human papilloma virus（HPV） infection in lesion tissues of patients with common anus and rectal disease .Methods Gene amplification combined with gene chip technology were employed to conduct genotyping test in lesion tissue of 566 patients with common anus and rectal disease .Results In lesion tissues of 566 patients with common anus and rectal disease ,the overall HPV infection rate was 32 .86% （186/566） .In male patients ,the overall HPV infection rate ,monopole infection rate and multiple infection rate were 32 .14% （117/364） ,23 .35% （85/364） and 8 .79% （32/364） ,respectively ,which showed no sta-tistically significant difference with female [34 .16% （69/202） ,24 .75% （50/202） and 9 .41% （19/202） ,respectively ] （P〉0 .05） . HPV 18 ,16 ,33 ,31 types were the main types of common anus and rectal disease .Conclusion HPV genotyping test of anus and rectum tissues is important for molecular epidemiological studies of HPV infection in anus and rectum .