不同方法检测乙型肝炎病毒表面抗原的结果分析

An analysis on results of different detection methods for hepatitis B virus surface antigen

目的分析金标免疫层析检测技术（GICA）、酶联免疫吸附测定（ELISA）和时间分辨免疫荧光分析（TRFIA）检测乙型肝炎病毒表面抗原（HBsAg）的结果。方法采用GICA、ELISA及TRFIA对11 058例标本进行HBsAg检测。结果 GICA、ELISA及TRFIA法检测11 058例标本HBsAg的阳性率分别为15.52%、15.64%及15.95%,两两比较,差异无统计学意义（P〉0.05）。TRFIA定量检测为0.2～〈2.0ng/mL时,GICA、ELISA的诊断符合率分别为25.0%、75.0%。结论检测HBsAg低浓度样本时,GICA和ELISA的诊断符合率降低。

Objective To analyze the results of gold labeled immunochromatographic assay （GICA ） ,enzyme-linked immunosor-bent assay （ELISA ） and time resolved fluoroimmunoassay （TRFIA ） detection for hepatitis B virus surface antigen （HBsAg ） . Methods GICA ,ELISA and TRFIA were employed to conduct the HBsAg testing in 11 058 specimens .Results The HBsAg posi-tive rates of GICA ,ELISA and TRFIA detection in 11 ,058 specimens were 15 .52% ,15 .64% and 15 .95% ,respectively ,with no statistically significant difference when pairwise comparison performed （P〉0 .05） .In specimens which results of TRFIA quantita-tive detection was 0 .2- 〈2 .0 ng/mL ,the diagnostic compliance rates of GICA ,ELISA were 25 .0% and 75 .0% ,respectively .Con-clusion The diagnostic compliance rates of GICA ,ELISA detection in specimens with low concentration HBsAg are deceased .