铁皮石斛抗肿瘤活性成分的探究

Extraction of Anti-tumor Constituents Derived from Dendrobium officinale

为了探究铁皮石斛中的抗肿瘤活性成分,本研究采用乙酸乙酯、95%乙醇、水三种不同极性的溶剂依次对铁皮石斛茎的干燥粉末进行了提取,初步得到了三种提取物;初提取物在MTT细胞增殖检测结果的指引下,结合硅胶柱层析、TLC薄层分析和凝胶柱层析进行分离纯化,最终筛选出具有强抗肿瘤活性的组分。结果显示乙酸乙酯提取物经分离纯化后得到了最强抗肿瘤活性成分,其对肝癌细胞HepG-2、胃癌细胞SGC-7901和乳腺癌细胞MCF-7的增殖都有明显的抑制效果,半抑制浓度IC50依次为:0.45μg/mL、0.36μg/mL、0.34μg/mL,经波谱学数据鉴定,该化合物为金钗石斛菲醌。金钗石斛菲醌首次分离自金钗石斛,其抗肿瘤功效已经得到证实,并引起了广泛的关注,本研究成果对以铁皮石斛为原料的药品和保健食品的开发具有重要的指导意义。

To explore the anti-tumor constituents from Dendrobium officinale,the components extracted with three solvents（ethyl acetate,95% ethanol and water） were separated and purified by silica gel column chromatography,TLC and Sephadex LH-20,and the whole process was guided by MTT method.Results showed that the strongest activity component named TD1 was achieved by the ethyl acetate extraction,the proliferation of human hapetocelluar carcinoma cell line（HepG-2）,human gastric carcinoma cell line（SGC 7901） and human breast cancer cell line（MCF-7） were significantly inhibited by TD1 and the IC50were 0.45 μg/mL,0.36 μg/mL and 0.34 μg/mL,respectively.TD1 was identified as denbinobin by spectroscopic data.Denbinobin was first isolated from Dendrobium nobile Lindl.and its anti-tumor effect has been certified and widely noted in recent years,the results may provide references for the development of new medicine and health food by using Dendrobium officinale as raw materials.