摘要
本文通过紫外吸收光谱法研究了落新妇苷与β-环糊精(β-CD)的包结反应。β-CD可与落新妇苷形成1:1包结物,并使其在291 nm处吸光度有规律下降,二者包结平衡常数为1788.54±273.41 M-1。采用研磨法制备落新妇苷与β-CD的包结物,考察了水分含量和摩尔比对落新妇苷包结率的影响。适当提高研磨过程中水份含量和β-CD摩尔比可增加落新妇苷包结率。最后,比较了β-CD包结物和落新妇苷单体的溶解度、体外溶出度和大鼠体内生物利用度。形成β-CD包结物可显著提高落新妇苷溶解度。25℃条件下,包结物中落新妇苷的溶解度为52.01 mM,为落新妇苷单独存在时的106.14倍。体外溶出度曲线表明包结物中落新妇苷的溶解速度更快。包结物中落新妇苷在大鼠体内达到峰值时间更短,且峰值浓度更高,但二者生物利用度相当,绝对生物利用度都约为3.7%。
The inclusion reaction of astilbin with β-cyclodextrin（β-CD） was investigated by UV spectrometry in this study.β-CD could form complex with astilbin in ratio of 1：1 with formation constant of 1788.54±273.41 M-1,and gradually decrease the absorbance of astilbin at 291 nm.The complex was prepared by co-grinding method.The effects of water content and molar ratio on inclusion rate during grind were investigated.The results showed that properly increasing of water content and β-CD molar ratio enhanced the formation of astilbin complexes.Finally,the solubility and in vivo bioavailability of astilbin and its β-CD complexes prepared with molar ratio of 1：2 were investigated.The solubility of astilbin in β-CD complexes was increased 106.14 times and its dissolution profiles were improved.The in vivo bioavailability study showed that β-CD enhanced the absorb rate of astilbin,shortened Tmax and increased Cmax.However,the absolute bioavailability between astilbin and its β-CD complexes had no difference,and were both around 3.7%.
出处
《现代食品科技》
EI
CAS
北大核心
2014年第5期82-86,共5页
Modern Food Science and Technology
基金
江西省自然科学基金资助项目(20122BAB214005)
江西农业大学青年基金资助项目(QN201108)
