针刺对慢性脑缺血脑皮质区自由基相关指标影响及其机制

Effect of Acupuncture on Indexes Related to Free Radicals in Cortex in Chronic Cerebral Ischemia Rats and Its Mechanism

目的:明确针刺对慢性脑缺血的脑皮质区自由基相关指标影响及其机制。方法:将实验大鼠随机分为正常、慢性脑缺血(IR)和针刺组,对比观察各组动物慢性脑缺血5周时脑皮质病理变化及超氧化物歧化酶(superoxide dismutase,SOD)、活性氧(reactive oxygen species,ROS)、丙二醛(malondialdehyde,MDA)、黄嘌呤氧化酶(xanthine oxidase,XOD)含量的影响。结果:IR组SOD值较正常组显著降低,针刺组的SOD活性表达明显高于IR组,ROS、XOD活性低于IR组,而MDA的活性明显低于IR组(P<0.05或P<0.01)。结论:针刺对慢性脑缺血有一定保护作用,此作用机制可能是针刺通过清除自由基,降低脂质过氧化应激而保护慢性脑缺血的神经功能损伤。

Objective：To explore the effect of acupuncture on indexes related to free radicals in cortex in chronic cerebral ischemia rats and its mechanism. Method： Rats were randomly divided into the control group, the chronic cerebral ischemia（IR） and the acupuncture group. The pathological change and the content of superoxide dismutase（SOD）,reactive oxygen species（ROS）,malondialdehyde（MDA）,xanthine oxidase（XOD） in cortex of rats were detected after five weeks. Result： The expression of SOD in the acupuncture group was significantly higher than that in group IR.The activity of SOD in the acupuncture group was significantly higher than that in IR group,and the content of ROS, MDA and XOD activity was all much lower than that in IR group（P〈0.05 or P〈0.01）. Conclusion： Acupuncture has protective effect on chronic cerebral ischemia by scavenging free radicals and reducing lipid peroxidation to relieve the neuron damage.