小麦品种(系)抗白粉病和Pm4b分子标记鉴定

Appraisal of Resistance to Powdery Mildew and Molecular Marker Analysis of Pm4b of Wheat Varieties( Lines)

采用室内苗期鉴定、大田人工接种鉴定和Pm4b分子标记对黄淮麦区的98个小麦品种(系)进行了白粉病抗性分析。苗期鉴定结果表明,被测品种中,抗病品种5个,占参试品种的5%。田间接种鉴定发现,抗病品种39个,占参试品种的39%。分子标记检测发现,98份供试材料中有3份样品检测到Pm4b基因的标记片段,占供试材料的3%。苗期感病而在成株期表现中等抗病有可能是慢病性品种,是一种更有利用前途的抗病种质。

In this study,the resistance to powdery mildew of 98 wheat varieties（ lines） of Huanghuai wheat growth area was analyzed using seedling identification,artificial inoculation identification in the field and molecular marker analysis of Pm4b. There only 5% tested varieties had resistance to powdery mildew by seedling stage identification. Field inoculation found 39 resistant varieties,accounting for 39% of the tested varieties. There were 3 samples,accounting for 3% of the tested materials,detected the gene marker fragment of Pm4b by molecular markers detected. These varieties which were susceptible in the seeding but disease-resistant in the adult stage were potential adult-plant resistance variety which was more promising in future disease-resistant breeding. This study provides an important basis for powdery mildew resistance breeding of wheat.