猪繁殖与呼吸综合征病毒Shanxi-6株全基因序列分析

Sequence Analysis of Whole Genome of Porcine Reproductive and Respiratory Syndrome Virus Shanxi-6 Strain

采用RT-PCR方法,分段扩增出猪繁殖与呼吸综合征病毒（PRRSV）Shanxi-6株15个基因片段,并将这15个片段分别连接到pMD18-T载体上进行克隆测序、拼接后,得到PRRSV Shanxi-6株全基因组长15 320 bp（不包含PolyA尾）,包含9个开放阅读框,5＇UTR长189 bp,3＇UTR长150 bp。基因组序列分析结果表明,Shanxi-6株与欧洲型代表毒株LV的核苷酸序列同源性为54.35%,与美洲型代表毒株VR-2332的核苷酸序列同源性为88.80%,与HPPRRSV毒株（JXA1、HUN4、HuN、HUB1）亲缘关系最近,核苷酸序列同源性高达97.44%-97.74%,且在非结构蛋白Nsp2上有30个氨基酸的缺失。

The fifteen gene fragments of Shanxi-6 strain of porcine reproductive and respiratory syndrome were amplified respectively by RT-PCR,cloned into pMD18-T vector and sequenced. The genome of Shanxi-6 strain in length was 15 320 bp excluding PloyA tail,containing 9 open reading frames（ ORFs）,the 5＇UTR in 189 bp and the 3＇UTR in 150 bp. The complete genomic analysis showed that Shanxi-6 strain shared 54. 35% of nucleotide homology with European genotype（ LV） and shared 88. 80% homology with American genotype（ VR-2332）. Shanxi-6 strain has correlative genetic relationship and shared 97. 44%- 97. 74% homology with highly pathogenic PRRSV（ JXA1,HUN4,HuN,HUB1）. 30 amino acids deletion was exhibited in Nsp2 protein of Shanxi-6 strain.