大白菜芜菁花叶病毒病抗性遗传及分子标记筛选

Inheritance of Resistance and Screening of Molecular Markers for Turnip Mosaic Virus in Chinese Cabbage

为了对大白菜芜菁花叶病毒病的抗性遗传规律进行研究,并筛选相关的分子标记,分别以高抗TuMV的高代自交系材料73和感TuMV的自交系材料06-247为亲本,构建其BC1群体,运用摩擦接种法以TuMV的C4株系对上述群体进行接种,同时采用生物学观察法进行抗病性鉴定,采用BSA法筛选相关基因的分子标记。研究结果表明,在该试验组合中,TuMV抗性是由一对隐性基因控制的,筛选出与该基因连锁的3个分子标记BrID90143(4.2 cM)、BrSSR4068(4.2 cM)和BrID10645(10.1 cM),为大白菜抗TuMV分子标记辅助选择及相关基因的精细定位奠定了基础。

In order to research the inheritance of TuMV resistance of Chinese cabbage（ Brassica campestris L. ssp. Pekinensis; genome AA,2n = 20） and screening molecular markers for related gene,we selected resistant material 73 and susceptible material 06-247 as parents respectively,and constructed the BC1（ F1× P1） generation. The above generations were inoculated with TuMV-C4 virus mechanically and TuMV resistance was tested by using the method of phenotype observation. We adopted BSA（ Bulked segregation analysis） method to screening molecular markers linked to TuMV resistance gene. It has been confirmed that in the group of 73 × 06-247,TuMV resistance was controlled by one pair of recessive genes and three molecular markers named BrID90143（ 4. 2 cM） and BrSSR4068（ 4. 2 cM） and BrID10645（ 10. 1 cM）,which was laid the foundation for breeding of TuMV resistant Chinese cabbage and further mapping this gene.