摘要
目的探讨氦-氖激光照射对体外培养神经元微管结合蛋(MAP2)表达的影响。方法采用无血清细胞培养方法,分离并体外培养新生大鼠海马神经元,并以低能量氦-氖激光对其照射,于培养后14d神经元生长旺盛时期取出各组培养皿内盖玻片进行MAP2免疫学组织化学染色,光镜下观察,拍照计数,与其他对照组比较,观察激光照射对神经元微管结合蛋白(MAP2)表达的影响。结果将氦-氖激光照射组与对照组比较,阳性表达细胞轮廓清晰,照射后阳性表达MAP2的培养神经元数目为(15.85±2.79)个/高倍视野,明显高于对照组[(9.23±2.56)个/高倍视野]的数量,且组间差异有统计学意义(P〈0.01)。结论氦-氖激光照射能促进体外培养新生大鼠海马神经元MAP2的表达。
Objective To study the effect of low energy He-Ne laser radiation on microtubule-associated protein 2 (MAP2) expression in cultured rat hippocampal neurons. Methods Neonatal rat hippocampal neurons were isolated and cultured in vitro. After 14 days culture with or without low energy He-Ne laser radiation, neurons were taken out; MAP2 expression was shown by using immunohistochemistry method and MAP2-positive neurons were counted on the photographs taken under light microscope. Results The amount of MAP2-positive neurons in laser radiation group was (15.85±2. 79)/high power field and was much higher than that in control group (9.23 ± 2.56). Positive expression neurons had clear contours. The difference of MAP2 expression between laser radiation group and control group was statistically significant ( P 〈 0.01 ). Expression of MAP2 was up-regulated after laser ra- diation in cultured neurons. Conclusions He-Ne laser radiation may up-regulate the expression of MAP2 in cul- tured neurons.
出处
《中华物理医学与康复杂志》
CAS
CSCD
北大核心
2014年第5期325-328,共4页
Chinese Journal of Physical Medicine and Rehabilitation
关键词
氦-氖激光
体外培养神经元
微管结合蛋白
He-Ne laser
Cultured neuron, in vitro
Mierotubule-assoeiated protein 2