低能量氦-氖激光照射对体外培养大鼠海马神经元微管结合蛋白表达的影响

Effect of low energy He-Ne laser radiation on microtubule-associated protein 2 expression in cultured rat hippocampal neurons

目的探讨氦-氖激光照射对体外培养神经元微管结合蛋（MAP2）表达的影响。方法采用无血清细胞培养方法，分离并体外培养新生大鼠海马神经元，并以低能量氦-氖激光对其照射，于培养后14d神经元生长旺盛时期取出各组培养皿内盖玻片进行MAP2免疫学组织化学染色，光镜下观察，拍照计数，与其他对照组比较，观察激光照射对神经元微管结合蛋白（MAP2）表达的影响。结果将氦-氖激光照射组与对照组比较，阳性表达细胞轮廓清晰，照射后阳性表达MAP2的培养神经元数目为（15．85±2．79）个／高倍视野，明显高于对照组[（9．23±2．56）个／高倍视野]的数量，且组间差异有统计学意义（P〈0．01）。结论氦-氖激光照射能促进体外培养新生大鼠海马神经元MAP2的表达。

Objective To study the effect of low energy He-Ne laser radiation on microtubule-associated protein 2 （MAP2） expression in cultured rat hippocampal neurons. Methods Neonatal rat hippocampal neurons were isolated and cultured in vitro. After 14 days culture with or without low energy He-Ne laser radiation, neurons were taken out; MAP2 expression was shown by using immunohistochemistry method and MAP2-positive neurons were counted on the photographs taken under light microscope. Results The amount of MAP2-positive neurons in laser radiation group was （15.85±2. 79）/high power field and was much higher than that in control group （9.23 ± 2.56）. Positive expression neurons had clear contours. The difference of MAP2 expression between laser radiation group and control group was statistically significant （ P 〈 0.01 ）. Expression of MAP2 was up-regulated after laser ra- diation in cultured neurons. Conclusions He-Ne laser radiation may up-regulate the expression of MAP2 in cul- tured neurons.