摘要
目的探讨微小RNA(miRNA)在胰腺癌患者血浆中的表达情况及临床意义。方法采用实时定量PCR方法检测30例胰腺癌和26例健康志愿者血浆中miR-21、miR-210、miR-155、miR.20a、miR-25和miR-196a的表达水平,并分析miR-21、miR-210、miR-155、miR-20a、miR-25和miR-196a与CA19-9联合诊断胰腺癌的价值。结果miR-21、miR-210、miR-155、miR-20a、miR-25和miR-196a在胰腺癌患者血浆中的中位表达水平分别为1.65×10^6、5.98×10^4、2.83×10^3、3.47×10^6、2.76×10^6和1.03×10^3 copies/μl,正常对照者分别为4.08×10^5、2.54×10^4、8.55×10^2、1.79×10^6、9.32×10^5和4.67×10^2 copies/μl,差异有统计学意义(P〈0.05)。miR-21、miR-210、miR-155、miR-20a、miR-25和miR-196a的受试者工作特征曲线(ROC曲线)下面积分别为0.893、0.810、0.820、0.766、0.816和0.729,miR-21的诊断价值最高。miR-155与miR-25联合诊断胰腺癌的ROC曲线下面积为0.913(95%口为0.838~0.988),明显优于单独miR-155和miR-25的诊断价值。miR-210与CA19-9、miR-25与CA19-9联合诊断胰腺癌的ROC曲线下面积分别为0.960(95%凹为0~1.0)和0.942(95%CI为0.876~1.0),优于单独CA19-9的ROC曲线下面积(0.862,95%CI为0.748~0.975)。miR-210、miR-25分别与CA19-9联合时诊断的灵敏度和准确率均明显升高。结论miR-21、miR-155、miR-25和miR-210对胰腺癌的诊断价值值得进一步研究。
Objective The aim of this study was to identify six miRNAs expressed in plasma of patients with pancreatic cancer (PCa) and analyze their value as a diagnostic index of pancreatic cancer. Methods Plasma total RNAs were extraeted from 30 PCa patients and 26 normal controls, and the abundance of six microRNAs was measured using real-time PCR. The possibility to combine them with CA19-9 as diagnostic biomarkers was analyzed. Results The expression level of miR-21, miR-210, miR- 155, miR-20a, miR-25 and miR-196a in plasma of patients with pancreatic cancer were 1.65 × 10^6, 5.98× 10^4, 2.83 × 10^3 , 3.47 × 10^6, 2.76 × 10^6, and 1.03 × 10^3 ( copies/μl) , while the normal controls were 4.08 ×10^5, 2.54 × 10^4, 8.55 × 10^2, 1.79 × 10^6, 9.32 × 10^5 , and 4.67 ×10^2 (copies/ixl) , respectively, with a significant difference between the two groups ( P 〈 0.05 ). The areas under the ROC curve of miR-21, miR-210, miR-155, miR-20a, miR-25 and miR-196a were 0. 893, 0. 810, 0. 820, 0. 766, 0. 816 and 0.729, respectively. MiR-21 had the highest diagnostic value when it was used as diagnostic marker alone. The combination of miR-155 and miR-25 was more effective to distinguish PCa from normal than to be used alone, and the area under the ROC curve was 0. 913 (95% CI 0. 838-0. 988 ). When CA199 associated with miR -210 and miR-25, respectively, the areas under the ROC curves were 0.96 (95% CI was 0-1.0)and 0.942(95% CI was 0. 876-1.0) , which were higher than CA199 alone (0. 862,95% CI was 0. 748- 0. 975 ). There was a high improvement in diagnostic sensitivity and accuracy when miR-210 and miR-25were combind with CA19-9, respectively. Conclusions Plasma miR-21, miR-155, miR-25, miR-210 have diagnostic value for pancreatic cancer, and deserve further study.
出处
《中华肿瘤杂志》
CAS
CSCD
北大核心
2014年第5期351-354,共4页
Chinese Journal of Oncology
基金
国家重点基础研究发展计划(2011CB911004)
科技北京百名领军人才培养工程(Z13110700513001)
北京市科技新星计划(Z13110700413066)
北京市自然科学基金(7122150)
关键词
胰腺肿瘤
血浆
微小RNA
诊断
Pancreatic neoplasms
Plasma
MicroRNA
Diagnosis