PDTC抑制NF-κB表达对兔蛛网膜下隙出血后脑血管重构的影响

Effect of PDTC-induced inhibition of NF-κB expression on cerebrovascular remodeling following subarachnoid hemorrhage

目的观察兔蛛网膜下隙出血模型基底动脉核因子κB(NF-κB)的表达及其对脑血管重构的影响。方法 16只雄性新西兰大白兔随机分为SAH组和吡咯烷二硫代氨基甲酸盐(PDTC)组,各8只。SAH组枕大池二次穿刺并注入自体动脉血造模,PDTC组在SAH后应用PDTC注入枕大池进行干预。模型制作完成后7 d处死动物,取其基底动脉测量平滑肌厚度。免疫组化法及蛋白质印迹法检测基底动脉中NF-κB、增殖细胞核抗原(PCNA)蛋白的表达。结果与SAH组相比,PDTC干预后,基底动脉中NF-κB与PCNA蛋白表达减少,平滑肌厚度变薄,与SAH组比较差异有统计学意义(P<0.05)。结论 PDTC可通过抑制NF-κB的激活减轻SAH后脑血管壁平滑肌增殖性改变。

Objective To observe the expression of NF-κβ in the basilar artery of rabbit model after subarachnoid hemorrhage （SAH） and its effect on cerebrovascular smooth muscle proliferation. Methods Sixteen male New Zealand rabbits were evenly randomized into two groups, ammonium pyrrolidinedithiocarbamate （PDTC） group and SAH group. Double injection of fresh autologous arterial blood into the cisterna magna was used to establish animal models of cerebral vasospasm. In PDTC group PDTC was injected each day into the cisterna magna in the model animals. The animals were sacrificed 7 days after model establishment; the smooth muscle thickness of basilar artery was observed; and expression of NF-κβ, proliferating cell nuclear antigen （PCNA） in the basilar artery was examined by immunohistochemistry and Western blotting analysis. Results Compared with SAH group, PDTC group had significantly decreased NF-κβ and PCNA protein expression and significantly reduced smooth muscle thickness （P〈0.05）. Conclusion PDTC can reduce the proliferation of smooth muscle through inhibiting the activation of NF-κβ after SAH in rabbits.